Urinary metallomics as a novel biomarker discovery platform: Breast cancer as a case study
Urinary metallomics as a novel biomarker discovery platform: Breast cancer as a case study
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DOI：
10.1016/j.cca.2015.11.014
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发表时间：
2016-01-15
影响因子：
5
通讯作者：
Bosnak, Cynthia P.
中科院分区：
文献类型：
--
作者：
Burton, Casey;Dan, Yongbo;Bosnak, Cynthia P.
背景资料：尿金属组学是一种新的“组学”的方法，旨在促进个性化的癌症筛查和预防，提高我们的理解，尿金属在diseases.Methods：22尿金属进行了检查与电感耦合等离子体质谱在138名新诊断为乳腺癌和良性疾病的妇女。从现场尿样中的尿金属调整到肾稀释using urine specific gravity.Results：两个尿金属，铜（P值= 0.036）和铅（P值= 0.003），在乳腺癌患者的尿液中显着增加。包括铜、铅和患者年龄的多变量模型提供了令人鼓舞的区分力（AUC：0.728，P值< 0.0005），而铜的单变量模型（61.7%的灵敏度，50.0%的特异性）和铅（76.6%的灵敏度，51.2%的特异性）在优化的截止阈值相比，与其他乳腺癌诊断方式，如乳房X线摄影有利。各种金属之间的相关性表明潜在的地理和饮食的影响，尿液metallome，值得进一步investigation.Conclusions：这个概念验证的工作介绍了尿金属组学作为一种非侵入性的，潜在的变革性的“组学”的方法，早期癌症检测。尿铜和铅也被初步确定为潜在的乳腺癌生物标志物。(C)2015 Elsevier B.V.版权所有。
Background: Urinary metallomics is presented here as a new "omics" approach that aims to facilitate personalized cancer screening and prevention by improving our understanding of urinary metals in disease.Methods: Twenty-two urinary metals were examined with inductively-coupled plasma-mass spectrometry in 138 women newly diagnosed with breast cancer and benign conditions. Urinary metals from spot urine samples were adjusted to renal dilution using urine specific gravity.Results: Two urinary metals, copper (P-value = 0.036) and lead (P-value = 0.003), were significantly increased in the urine of breast cancer patients. A multivariate model that comprised copper, lead, and patient age afforded encouraging discriminatory power (AUC: 0.728, P-value < 0.0005), while univariate models of copper (61.7% sensitivity, 50.0% specificity) and lead (76.6% sensitivity, 51.2% specificity) at optimized cutoff thresholds compared favorably with other breast cancer diagnostic modalities such as mammography. Correlations found among various metals suggested potential geographic and dietary influences on the urine metallome that warrant further investigation.Conclusions: This proof-of-concept work introduces urinary metallomics as a noninvasive, potentially transformative "omics" approach to early cancer detection. Urinary copper and lead have also been preliminarily identified as potential breast cancer biomarkers. (C) 2015 Elsevier B.V. All rights reserved.