Investigation on ultrasound assisted extraction of saikosaponins from Radix Bypleuri
Investigation on ultrasound assisted extraction of saikosaponins from Radix Bypleuri
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DOI：
10.1016/j.seppur.2006.12.002
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发表时间：
2007-07-01
影响因子：
8.6
通讯作者：
Liang, Hanhua
中科院分区：
文献类型：
--
作者：
Zhao, Shuna;Kwok, Kin-Chor;Liang, Hanhua
研究了超声波辅助提取柴胡皂苷的工艺条件。考察了时间（t）、温度（7）、超声功率（1）、粒径（S）、溶剂固比（R）和溶剂浓度（C）等因素对目标组分收率的影响。实验结果表明，超声波辅助萃取的萃取速度是常规热萃取的6倍。还发现，在超声处理的前30分钟内获得了快速的提取速率。延长的超声处理没有使产率成比例增加。对于超声功率的影响，在21 W左右的功率水平下获得最大产量。获得UAE处理后植物细胞的扫描电子显微镜（SEM）图像，以提供躯体化效应的视觉证据。讨论了粒度、溶剂浓度、溶剂固比等因素对产率的影响。初步确定了柴胡皂苷超声乳化的最佳工艺条件为：超声乳化时间30 min，温度80 ℃，功率21 W，粒径< 0.3 mm，液固比25 ml/g，溶剂浓度50%。(c)2007 Elsevier B. V.保留所有权利。
Ultrasound assisted extraction (UAE) of saikosaponins from Radix Bupleuri (Bupleurum Chinense DC) was studied. The effects of various factors such as time (t), temperature (7), ultrasound power (1), particle size (S), solvent to solid ratio (R) and solvent concentration (C) on the yield of target components were investigated. The experimental results indicated that the extraction by UAE is six times faster than those by conventional thermal extraction. It is also found that fast extraction rate was obtained in the first 30 min of sonication. Prolonged sonication did not give a proportional increase in the yield. For the effect of ultrasound power, the maximum yield was obtained at the power level of around 21 W. Scanning electron microscope (SEM) images of the plant cells after UAE treatment were obtained to provide visual evidence of the somication effect. The effects of particle size, solvent to solid ratio and solvent concentration on the yield are also discussed. The preliminary optimum conditions for UAE of saikosaponins were found at time of 30 min, temperature of 80 degrees C, power of 21 W, particle size < 0.3 mm, solvent to solid ratio of 25 ml/g and solvent concentration of 50%. (c) 2007 Elsevier B.V. All rights reserved.