IMMUNOLOGICAL DETECTION OF CELL-SURFACE COMPONENTS RELATED WITH AGGREGATION OF CHINESE-HAMSTER AND CHICK EMBRYONIC-CELLS
IMMUNOLOGICAL DETECTION OF CELL-SURFACE COMPONENTS RELATED WITH AGGREGATION OF CHINESE-HAMSTER AND CHICK EMBRYONIC-CELLS
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DOI：
10.1016/0012-1606(79)90017-4
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发表时间：
1979-01-01
影响因子：
2.7
通讯作者：
TAKEICHI, M
中科院分区：
文献类型：
--
作者：
URUSHIHARA, H;OZAKI, HS;TAKEICHI, M
中国仓鼠V79细胞的相互粘附机制有两种，即Ca ~（2+）依赖性和Ca ~（2+）非依赖性。在这里，通过用含Ca 2+的胰蛋白酶（0.01%）分散细胞单层来制备仅具有完整的Ca 2+依赖性机制的细胞。在不存在Ca 2+的情况下，用非常低的胰蛋白酶浓度（0.0001%）分散的细胞仅保留完整的Ca 2+非依赖性机制。针对V79细胞表面抗原的Fab抗体片段通过Ca 2+非依赖性机制抑制V79细胞的聚集，但不通过Ca 2+依赖性机制抑制这些细胞的聚集。这两种细胞粘附机制可能基于不同的细胞组分。负责Ca 2+非依赖性粘附机制的分子可能是细胞表面组分，因为它们通过0.01%胰蛋白酶处理从细胞中释放而不失去其特异性抗原性。在鸡胚神经视网膜细胞中发现了与V79细胞相似的粘附机制。这些细胞粘附机制通常存在于各种细胞类型中。
Chinese hamster V79 cells were previously suggested to possess 2 mechanisms for their mutual adhesion, Ca2+-dependent and Ca2+-independent ones. Here cells with only the Ca2+-dependent mechanism intact were prepared by dispersing cell monolayers with trypsin (0.01%) containing Ca2+. Cells dispersed with a very low trypsin concentration (0.0001%) in the absence of Ca2+ retain only the Ca2+-independent mechanism intact. Fab antibody fragments directed against surface antigens of V79 cells inhibited the aggregation of V79 cells by the Ca2+-independent mechanism, but did not inhibit the aggregation of these cells by the Ca2+-dependent mechanism. The 2 cell adhesion mechanisms may be based on different cellular components. Molecules responsible for the Ca2+-independent adhesion mechanism are probably cell surface components, because they were released from cells by the treatment with 0.01% trypsin without losing their specific antigenicity. The presence of adhesion mechanisms similar to those in V79 cells was shown in neural retinal cells of chick embryos. These mechanisms of cell adhesion supposedly are generally present among a variety of cell types.