Bcl-2 protein expression during murine development.
Bcl-2 protein expression during murine development.
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发表时间：
1994-07
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通讯作者：
D. Novack;S. Korsmeyer
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作者：
D. Novack;S. Korsmeyer
Bcl-2在进化上保守的细胞死亡途径中作为死亡阻遏分子发挥作用。为了进一步探讨Bcl-2在发育中的作用，我们评估了其在小鼠胚胎发生过程中的表达模式。免疫组织化学分析表明，Bcl-2是广泛表达的小鼠胚胎发育早期的组织来源于所有三个胚层，这种表达成为限制与成熟。在上皮内，E12.5肺芽显示Bcl-2表达的近端至远端梯度，其被E18.5增强。Bcl-2在E14.5之前在整个肠上皮中表达，但到E18.5时，只有隐窝和较低绒毛中的细胞表达Bcl-2。在中胚层来源的肾脏中，Bcl-2在E12.5时在输尿管芽和后肾帽组织中表达。管状结构也表达Bcl-2，尽管总体水平随着肾脏成熟而下降。视网膜神经上皮细胞均匀表达Bcl-2，直到细胞开始分化，然后显示维持到成年期的地形分布。发育中的肢体提供了一个明确的例子，其中Bcl-2仅限于细胞存活区; Bcl-2在趾区表达，但不在细胞死亡的趾间区。Bcl-2在发育中的小鼠中的广泛分布表明许多未成熟细胞需要死亡阻遏物分子，或者Bcl-2可能具有超出调节发育细胞死亡的作用。
Bcl-2 functions as a death repressor molecule in an evolutionarily conserved cell death pathway. To further explore the role of Bcl-2 in development, we assessed its pattern of expression during murine embryogenesis. Immunohistochemical analysis demonstrates that Bcl-2 is widely expressed early in mouse fetal development in tissues derived from all three germ layers and that this expression becomes restricted with maturation. Within epithelium, the E12.5 lung bud demonstrates a proximal to distal gradient of Bcl-2 expression which is enhanced by E18.5. Bcl-2 is expressed throughout the intestinal epithelium through E14.5, but by E18.5 only cells in the crypts and lower villi express Bcl-2. In the mesoderm-derived kidney, Bcl-2 is expressed in both the ureteric bud and metanephric cap tissue at E12.5. Tubular structures also express Bcl-2, although overall levels drop as the kidney matures. Retinal neuroepithelial cells uniformly express Bcl-2 until cells begin to differentiate and then display the topographic distribution maintained into adulthood. The developing limb provides a clear example where Bcl-2 is restricted to zones of cell survival; Bcl-2 is expressed in the digital zones but not in the interdigital zones of cell death. The wide distribution of Bcl-2 in the developing mouse suggests that many immature cells require a death repressor molecule or that Bcl-2 may have roles beyond regulating developmental cell death.