A novel complex recombinant form of type 48-related human adenovirus species D isolated in Japan.
A novel complex recombinant form of type 48-related human adenovirus species D isolated in Japan.
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DOI：
10.7883/yoken.67.282
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发表时间：
2014
影响因子：
2.2
通讯作者：
T. Fujimoto;Shotaro Yamane;T. Ogawa;Nozomu Hanaoka;Atsushi Ogura;Chiemi Hotta;T. Niwa;Yakou Chiba;G. González;K. Aoki;Kanako O. Koyanagi;Hidemi Watanabe
中科院分区：
文献类型：
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作者：
T. Fujimoto;Shotaro Yamane;T. Ogawa;Nozomu Hanaoka;Atsushi Ogura;Chiemi Hotta;T. Niwa;Yakou Chiba;G. González;K. Aoki;Kanako O. Koyanagi;Hidemi Watanabe
最近，已经报道了人腺病毒（HAdV）的新基因型，并且已经发现它们中的许多是不同已知类型的HAdV种类D（HAdV-D）的重组形式。本研究的目的是记录从日本结膜炎患者的眼拭子中获得的新型分离株（HAdV_Chiba_E086/2012）的进化特征。从分离物中提取病毒DNA，通过桑格法对全基因组进行测序，并与HAdV-Ds的可用基因组序列进行比对。HAdV_Chiba_E086/2012的五邻体碱基、六邻体和纤维基因以及E3区的核苷酸序列的系统发育树显示，HAdV_Chiba_E086/2012与HAdV基因型65最接近（HAdV-GT 65）、HAdV-48、HAdV-GT 60和HAdV-22的同源性分别为98%、99%、95%和98%，提示该分离物是一种新的重组形式，命名为P65 H48 F60。进一步的系统发育和重组分析的基因组比对的新分离物暗示，嵌套重组事件涉及HAdV-GT 59，GT 65，48，GT 60，22，和一些祖先谱系或其近亲塑造了它的基因组。这些结果表明，HAdV_Chiba_E086/2012是在日本发现的第一个与HAdV-48相关的HAdV，其在迄今为止已知的HAdV中具有最复杂的进化历史。
Recently, new genotypes of human adenoviruses (HAdVs) have been reported and many of them have been found to be recombinant forms of different known types of HAdV species D (HAdV-D). The objective of this study was to document the evolutionary features of a novel isolate (HAdV_Chiba_E086/2012) obtained from the eye swab of a patient with conjunctivitis in Japan. Viral DNA was extracted from the isolate to sequence the whole genome by the Sanger method and aligned with available genome sequences of HAdV-Ds. The phylogenetic trees of the nucleotide sequences of the penton base, hexon, and fiber genes and the E3 region showed that HAdV_Chiba_E086/2012 is closest to HAdV genotype 65 (HAdV-GT65), HAdV-48, HAdV-GT60 and HAdV-22 at 98%, 99%, 95% and 98% identity, respectively, suggesting that this isolate is a novel recombinant form to be designated as P65H48F60. Further phylogenetic and recombination analyses of the genome alignment of the new isolate implied that nested recombination events involving HAdV-GT59, GT65, 48, GT60, 22, and some ancestral lineages or their close relatives have shaped its genome. These results showed that HAdV_Chiba_E086/2012 is the first HAdV-48-related HAdV found in Japan, which has the most complicated evolutionary history among the known HAdVs so far.