Marrow-derived CD40-positive cells are required for mice to clear Cryptosporidium parvum infection.
Marrow-derived CD40-positive cells are required for mice to clear Cryptosporidium parvum infection.
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小鼠需要骨髓来源的 CD40 阳性细胞来清除小隐孢子虫感染。
DOI：
10.1128/iai.69.3.1630-1634.2001
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发表时间：
2001
影响因子：
3.1
通讯作者：
Ponnuraj,EM
中科院分区：
文献类型：
--
作者：
Hayward,AR;Cosyns,M;Jones,M;Ponnuraj,EM
为了清除隐孢子虫感染，小鼠需要CD 4 +T细胞，主要组织相容性复合物II类和完整的CD 40-CD 154信号通路。CD 40在骨髓来源的细胞如树突状细胞和B淋巴细胞上组成型表达，并且在大多数体细胞上由γ干扰素（IFN-γ）诱导。确定CD 40是否需要清除aC。由于寄生虫感染必须发生在骨髓来源的单核细胞或通常携带寄生虫的上皮细胞上，我们将CD 40 +/−小鼠移植了CD 40 +/−骨髓，然后用C.小的这些嵌合体清除了C。而移植了CD 40 −/−骨髓细胞的CD 40 +/−对照组仍然受到感染。因此，骨髓源性细胞上的CD 40表达足以用于aC。肠上皮细胞CD 40表达不足。C.肠系膜淋巴结T细胞对CD 69和CD 154的获得无差异。具有完整或破坏的CD 40-CD 154通路的细小病毒感染动物。CD 4 T细胞进入C.无论这些受体的CD 40基因是否完整。这些结果表明，对于aC。在需要清除细小病毒感染的情况下，CD 40不是T细胞活化所必需的，而是可能有助于骨髓源性细胞的效应途径。
To clear aCryptosporidium parvuminfection, mice need CD4+T cells, major histocompatibility complex class II, and an intact CD40-CD154 signaling pathway. CD40 is constitutively expressed on marrow-derived cells such as dendritic cells and B lymphocytes and is induced by gamma interferon (IFN-γ) on most somatic cells. To determine whether the CD40 needed to clear aC. parvuminfection has to be on marrow-derived mononuclear cells or on the epithelial cells that normally harbor the parasite, we transplanted CD40−/−mice with CD40+/−bone marrow and then infected them withC. parvum. These chimeras cleared theC. parvuminfection, while CD40+/−controls transplanted with CD40−/−marrow cells remained infected. CD40 expression on marrow-derived cells therefore suffices for aC. parvuminfection to be cleared, while CD40 expression on intestinal epithelial cells is not sufficient. There was no difference between the acquisition of CD69 and CD154 by mesenteric lymph node T cells ofC. parvum-infected animals with intact or disrupted CD40-CD154 pathways. CD4 T cells entered the intestinal laminae propriae ofC. parvum-infected animals whether or not the CD40 genes of these recipients were intact. These results suggest that, for aC. parvuminfection to be cleared, CD40 is not necessary for T-cell activation but may instead contribute to an effector pathway of marrow-derived cells.