Molecular signature of hypersaline adaptation: insights from genome and proteome composition of halophilic prokaryotes.
Molecular signature of hypersaline adaptation: insights from genome and proteome composition of halophilic prokaryotes.
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高盐适应的分子特征：基因组的见解和卤素原核生物的蛋白质组组成。
DOI：
10.1186/gb-2008-9-4-r70
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发表时间：
2008-04-09
期刊：
影响因子：
12.3
通讯作者：
Dutta C
中科院分区：
文献类型：
--
作者：
Paul S;Bag SK;Das S;Harvill ET;Dutta C
嗜盐和非嗜盐原核生物的比较基因组和蛋白质组研究鉴定了嗜盐物种典型的特定基因组和蛋白质组特征，这些特征独立于基因组GC含量和分类位置。嗜盐原核生物适合在极端盐度条件下繁衍生息。对嗜盐菌不同大分子特征的鉴定和分析可以深入了解其适应高盐环境的因素。本报告对嗜盐和非嗜盐微生物的基因组和蛋白质组组成进行了广泛和系统的比较分析，以期确定盐适应的大分子特征。对嗜盐菌和非嗜盐菌的基因组和蛋白质组的比较分析揭示了嗜盐菌的一些共同趋势，这些趋势超越了物种的系统发育关系和基因组GC含量的界限。在蛋白质水平上，嗜盐物种的特征是疏水性低、酸性残基（尤其是天冬氨酸）过多、半胱氨酸（Cys）代表性不足、螺旋形成倾向较低和卷曲结构倾向较高。在DNA水平上，嗜盐基因组的二核苷酸丰度谱具有一些共同特征，这些特征与非嗜盐基因组截然不同，因此可以被视为盐适应的特定基因组特征。无论其长期进化历史如何，嗜盐菌中的同义密码子使用也表现出相似的模式。本研究证明了极端喜盐生物体环境适应的分子特征的普遍性，主张嗜盐物种向特定的基因组和氨基酸组成趋同进化，而不管其不同的GC偏向和广泛不同的分类位置。嗜盐生物的适应性特征似乎与控制 DNA 和蛋白质稳定性的物理原理有关，以应对它们繁衍生息的极端环境条件。
A comparative genomic and proteomic study of halophilic and non-halophilic prokaryotes identifies specific genomic and proteomic features typical of halophilic species that are independent from genomic GC-content and taxonomic position. Halophilic prokaryotes are adapted to thrive in extreme conditions of salinity. Identification and analysis of distinct macromolecular characteristics of halophiles provide insight into the factors responsible for their adaptation to high-salt environments. The current report presents an extensive and systematic comparative analysis of genome and proteome composition of halophilic and non-halophilic microorganisms, with a view to identify such macromolecular signatures of haloadaptation. Comparative analysis of the genomes and proteomes of halophiles and non-halophiles reveals some common trends in halophiles that transcend the boundary of phylogenetic relationship and the genomic GC-content of the species. At the protein level, halophilic species are characterized by low hydrophobicity, over-representation of acidic residues, especially Asp, under-representation of Cys, lower propensities for helix formation and higher propensities for coil structure. At the DNA level, the dinucleotide abundance profiles of halophilic genomes bear some common characteristics, which are quite distinct from those of non-halophiles, and hence may be regarded as specific genomic signatures for salt-adaptation. The synonymous codon usage in halophiles also exhibits similar patterns regardless of their long-term evolutionary history. The generality of molecular signatures for environmental adaptation of extreme salt-loving organisms, demonstrated in the present study, advocates the convergent evolution of halophilic species towards specific genome and amino acid composition, irrespective of their varying GC-bias and widely disparate taxonomic positions. The adapted features of halophiles seem to be related to physical principles governing DNA and protein stability, in response to the extreme environmental conditions under which they thrive.
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DOI：
10.1093/dnares/dsm015
发表时间：
2007-08-31
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Bag SK;Paul S;Ghosh S;Dutta C
通讯作者：
Dutta C
影响因子：
5.6
作者：
Fleming, PJ;Richards, FM
通讯作者：
Richards, FM
影响因子：
56.9
作者：
DYM, O;MEVARECH, M;SUSSMAN, JL
通讯作者：
SUSSMAN, JL
影响因子：
11.4
作者：
HO, PS;ELLISON, MJ;RICH, A
通讯作者：
RICH, A
影响因子：
7
作者：
Baliga, NS;Bonneau, R;Ng, WV
通讯作者：
Ng, WV