Localization of platelet antigens in human kidney disease.
Localization of platelet antigens in human kidney disease.
复制标题
血小板抗原在人类肾脏疾病中的定位。
DOI：
10.1038/ki.1980.160
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发表时间：
1980
影响因子：
19.6
通讯作者：
Michael,AF
中科院分区：
文献类型：
--
作者：
Miller,K;Dresner,IG;Michael,AF
方法血小板分离。通过修改 Zim-merman 和 Kolb 的方法将血小板与其他细胞分离[3]。将从明尼苏达大学医院血库获得的汇集的人类 0、Rh 阴性血小板浓缩液 (200 ml) 以 x620g 离心 10 mm。将富含血小板的上清液（红细胞和淋巴细胞含量低于 0.1%）在 x2000g 下离心 10 mm，用 30ml 含有 0.02M 磷酸钠的 0. 145-M 氯化钠溶液（pH，7.40；PBS）洗涤 3 次，并在 x2000g 下离心回收。然后将血小板沉淀悬浮在 5.0 ml PBS 中，并在硅化管中分成 0.5 ml 等份，在干冰-丙酮混合物中快速冷冻，并保存在 7°0 C 下直至使用。
MethodsPlatelet isolation. Platelets were separated from other cells by a modification of the method of Zim-merman and Kolb [3]. Pooled human 0, Rh-nega-tive platelet concentrate (200 ml) obtained from the blood bank at the University of Minnesota Hospi-tals, was centrifuged at x620g for 10 mm. The platelet-rich supernate, containing less than 0.1% erythrocyte and lymphocytes, was centrifuged at x2000g for 10 mm, washed three times in 30 ml of a 0. 145-M sodium chloride solution containing 0.02 M sodium phosphate (pH, 7.40; PBS), and was recovered by centrifugation at x 2000g. The platelet pellet was then suspended in 5.0 ml of PBS and separated into 0.5-mI aliquots in siliconized tubes, quick-fro-zen in a dry ice-acetone mixture, and stored at _7Ø0 C until used.