Negotiation of Intracellular Membrane Barriers by TAT-Modified Gold Nanoparticles
Negotiation of Intracellular Membrane Barriers by TAT-Modified Gold Nanoparticles
复制标题
DOI：
10.1021/nn201369k
复制
发表时间：
2011-06-01
期刊：
影响因子：
17.1
通讯作者：
Brust, Mathias
中科院分区：
文献类型：
--
作者：
Krpetic, Zeljka;Saleemi, Samia;Brust, Mathias
本文有助于讨论纳米物体如何在生物细胞内通过膜屏障。利用原子发射光谱法对HeLa细胞对肽修饰金纳米颗粒的摄取进行了定量研究。来自HIV病毒的TAT肽被认为是一种特别有效的细胞摄取启动子。利用透射电镜和系统图像分析技术，详细研究了tat修饰金纳米颗粒细胞内分布随时间的变化规律。观察到颗粒从细胞质和细胞核中消失并大量积聚在泡体中的不寻常趋势。通过膜破裂和通过膜边界的直接转移，经常发现颗粒的随后释放。最终，几乎完全清除了细胞中的颗粒。这项工作为细胞穿透肽可以使小物体在没有专用运输机制的情况下通过膜屏障的假设提供了支持。
This paper contributes to the debate on how nanosized objects negotiate membrane barriers inside biological cells. The uptake of peptide-modified gold nanoparticles by HeLa cells has been quantified using atomic emission spectroscopy. The TAT peptide from the HIV virus was singled out as a particularly effective promoter of cellular uptake. The evolution of the intracellular distribution of TAT-modified gold nanoparticles with time has been studied in detail by TEM and systematic image analysis. An unusual trend of particles disappearing from the cytosol and the nucleus and accumulating massively in vesicular bodies was observed. Subsequent release of the particles, both by membrane rupture and by direct transfer across the membrane boundary, was frequently found. Ultimately, near total clearing of particles from the cells occurred. This work provides support for the hypothesis that cell-penetrating peptides can enable small objects to, negotiate membrane barriers also in the absence of dedicated transport mechanisms.