A novel class of small RNAs in mouse spermatogenic cells
A novel class of small RNAs in mouse spermatogenic cells
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DOI：
10.1101/gad.1434406
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发表时间：
2006-07-01
影响因子：
10.5
通讯作者：
Lin, Haifan
中科院分区：
文献类型：
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作者：
Grivna, Shane T.;Beyret, Ergin;Lin, Haifan
小的非编码RNA，包括小干扰RNA(SiRNAs)和长度与21个核苷酸(NT)相似的微小RNAs(MiRNAs)，在不同的生物体中已经成为转录和转录后水平上基因表达的有效调节因子。在这里，我们报告了在小鼠雄性生殖系中鉴定出一类新的小RNA，称为piwi相互作用RNAs(PiRNAs)。PiRNAs的长度类似于30个核苷酸。它们在精子发生过程中表达，主要在精子细胞中表达。PiRNAs与精子发生特异性PIWI亚家族成员MIWI相关，依赖于MIWI的生物发生和/或稳定性。此外，piRNAs的一个亚群与多聚体相关，这表明它们在翻译调控中可能发挥作用。
Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of similar to 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class of small RNAs in the mouse male germline termed piwi-interacting RNAs (piRNAs). piRNAs are similar to 30 nt in length. They are expressed during spermatogenesis, mostly in spermatids. piRNAs are associated with MIWI, a spermatogenesis-specific PIWI subfamily member of the Argonaute protein family, and depend on MIWI for their biogenesis and/or stability. Furthermore, a subpopulation of piRNAs are associated with polysomes, suggesting their potential role in translational regulation.