Connexin expression in the developing avian cardiovascular system.
Connexin expression in the developing avian cardiovascular system.
复制标题
连接蛋白在发育中的禽类心血管系统中的表达。
DOI：
10.1161/01.res.73.1.71
复制
发表时间：
1993
影响因子：
20.1
通讯作者：
Hertzberg,EL
中科院分区：
文献类型：
--
作者：
Minkoff,R;Rundus,VR;Parker,SB;Beyer,EC;Hertzberg,EL
最近的观察表明，差距连接蛋白connexin 43在禽类胚胎发育中的心血管系统中的表达模式与以前在哺乳动物物种中观察到的模式显著不同。因此，通过使用两种不同的连接蛋白43特异性抗肽抗体的免疫荧光定位以及Western和北方印迹分析，对鸡胚胎中连接蛋白43的表达进行了详细的分析。在禽类心肌、静脉系统或动脉系统的较小血管中未检测到连接蛋白43蛋白。相反，它仅限于动脉流出道的血管，在胚胎第8天变得明显的同心模式。用抗α-平滑肌肌动蛋白和连接蛋白43的双重染色证明了在流出道血管壁的介质中的共定位。连接蛋白43的发育表达被发现反映了次级肌动蛋白的空间模式;然而，连接蛋白43先于次级肌动蛋白的表达1-2天。与此相反，相关的间隙连接蛋白（连接蛋白42）的抗体显示在禽流出道免疫染色的情况下。用抗连接蛋白42和抗A细胞粘附分子（对禽类闰盘特异性）的双重染色证明了心肌细胞之间的共定位，表明连接蛋白42是禽类心肌间隙连接的组成部分。鉴于这些发现，不同的心肌连接蛋白的发育表达可能调和以前的研究表明，不同的生理特性的鸟类和哺乳动物的心脏间隙连接。
Recent observations have suggested that the patterns of expression of the gap junction protein connexin43 in the developing cardiovascular system of the avian embryo diverge significantly from the patterns previously seen in mammalian species. Therefore, a detailed analysis of connexin43 expression in the chicken embryo was performed by use of immunofluorescent localization with two different connexin43-specific antipeptide antibodies as well as Western and Northern blot analysis. Connexin43 protein was not detected in the avian myocardium, the venous system, or the smaller vessels of the arterial system. Rather, it was limited exclusively to the vessels of the arterial outflow tract in a concentric pattern that became evident by embryonic day 8. Double staining with anti-alpha-smooth muscle actin and connexin43 demonstrated colocalization in the media of outflow tract vessel walls. The developmental expression of connexin43 was found to mirror the spatial patterning of secondary actin; connexin43, however, preceded the expression of secondary actin by a period of 1-2 days. In contrast, antibodies to a related gap junction protein (connexin42) revealed an absence of immunostaining in the avian outflow tract. Double staining with anti-connexin42 and anti-A-cell adhesion molecule (specific for avian intercalated discs) demonstrated colocalization between cardiac myocytes, indicating that connexin42 is a constituent of avian myocardial gap junctions. In light of these findings, developmental expression of differing myocardial connexins may reconcile previous studies showing different physiological properties of avian and mammalian cardiac gap junctions.