A smartphone integrated paper (SIP)-based platform for rapid and on-site screening of urinary tract infections
A smartphone integrated paper (SIP)-based platform for rapid and on-site screening of urinary tract infections
复制标题
基于智能手机集成纸质 (SIP) 的平台，用于快速现场筛查尿路感染
DOI：
10.1016/j.snb.2023.133498
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Park, Sung-Yong
中科院分区：
文献类型：
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作者：
Janev, Athul;Kang, John S.;Park, Sung-Yong
我们提出了一种基于智能手机集成纸（SIP）的系统，可以通过直接识别人类尿液样本中的白细胞（wbc）来准确筛查尿路感染（uti）。SIP平台主要由纸质毛细管滤镜和集成智能手机的荧光显微镜组成。纸过滤装置是一种低成本，简单的方法，可以选择性地染色和分离尿液中的目标白细胞，收集效率超过95%，使用毛细管作用通过具有几种不同孔径的纸层，而无需额外的泵和管，通常需要在微流体系统中进行流体驱动。此外，集成的智能手机可以方便地用于染色白细胞的荧光成像和显微镜分析，从而在护理点快速提供UTI诊断结果，而无需笨重的实验室设备和专业培训。SIP平台的制作工艺简单，成本效益高，有利于资源有限的现场应用。我们通过实验证明了SIP能够使用纸质毛细管滤镜分离尿液溶液中的目标白细胞（选择性超过95%），智能手机显微镜在约12.25 mm2的视野范围内以14.4倍的放大倍率对尿液溶液进行荧光成像的有效性，平台细胞计数的可靠性为96.67%的灵敏度和100%的特异性。以及成功区分尿路感染患者和健康人的真实尿液样本。从过滤到白细胞定量的整个筛选过程在10分钟内迅速完成，显示了其在护理点应用的潜力。SIP技术提供了一种低成本、快速和便携的尿路感染诊断工具，可以改善发展中地区获得准确检测结果的机会，从而通过加快治疗时间减少痛苦。
We present a smartphone integrated paper (SIP)-based system that enables accurate screening of urinary tract infections (UTIs) by directly identifying white blood cells (WBCs) in human urine samples. The SIP platform mainly consists of a paper-based capillaric filter and a smartphone-integrated fluorescence microscope. A paper filtration device allows a low-cost, simple method for selectively dying and isolating target WBCs in urine with more than 95% collection efficiency using capillary action through paper layers with several different pore sizes without extra pumps and tubes typically required for fluidic actuation in microfluidic systems. Furthermore, an integrated smartphone can be conveniently used for fluorescence imaging of the stained WBCs and microscopic analysis to rapidly provide UTI diagnostic results at the point of care without the need of bulky laboratory equipment and specialized training. The fabrication process for the SIP platform is simple and cost-effective, being beneficial for resource-limited field applications. We have experimentally demonstrated the capability of the SIP for isolation of target WBCs in a urine solution (more than 95% selectivity) using the paper capillaric filter, the effectiveness of the smartphone microscope for fluorescence imaging of urine solutions with 14.4 × magnification over a field of view of ∼12.25 mm2, the reliability of the platform’s cell counting with the 96.67% sensitivity and 100% specificity, and successful differentiation of the real urine samples between UTI patient and healthy person. The entire screening processes from filtration to quantification of WBCs in real human urine samples were rapidly completed within 10 min, showing its potential for point-of-care applications. The SIP technology provides a low-cost, rapid, and portable UTI diagnostic tool that can improve the access of accurate test results to developing areas and thus reduce suffering by speeding up therapy timelines.