Protection from lethal challenge in a neonatal mouse model by circulating recombinant form coxsackievirus A16 vaccine candidates.
Protection from lethal challenge in a neonatal mouse model by circulating recombinant form coxsackievirus A16 vaccine candidates.
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通过循环重组柯萨奇病毒 A16 候选疫苗保护新生小鼠模型免受致命挑战
DOI：
10.1099/vir.0.063560-0
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Yu XF
中科院分区：
文献类型：
--
作者：
Li J;Chang J;Liu X;Yang J;Guo H;Wei W;Zhang W;Yu XF
Circulating coxsackievirus A16 (CA16) is a major cause of hand, foot and mouth disease (HFMD) in South-east Asia. At present, there is no vaccine against CA16. Pathogenic animal models that are sensitive to diverse circulating CA16 viruses would be desirable for vaccine development and evaluation. In this study, we isolated and characterized several circulating CA16 viruses from recent HFMD patients. These CA16 viruses currently circulating in humans were highly pathogenic in a newly developed neonatal mouse model; we also observed and analysed the pathogenesis of representative circulating recombinant form CA16 viruses. An inactivated CA16 vaccine candidate, formulated with alum adjuvant and containing submicrogram quantities of viral proteins, protected neonatal mice born to immunized female mice from lethal-dose challenge with a series of CA16 viruses. Further analysis of humoral immunity showed that antibody elicited from both the immunized dams and their pups could neutralize various lethal viruses by a cytopathic effect in vitro. Moreover, viral titres and loads in the tissues of challenged pups in the vaccine group were far lower than those in the control group, and some were undetectable. This lethal-challenge model using pathogenic CA16 viruses and the vaccine candidates that mediated protection in this model could be useful tools for the future development and evaluation of CA16 vaccines.
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影响因子：
5.5
作者：
Chen, Chun-Wei;Lee, Yi-Ping;Yu, Chun-Keung
通讯作者：
Yu, Chun-Keung
DOI：
10.1016/s1684-1182(10)60043-6
发表时间：
2010-08-01
影响因子：
7.4
作者：
Wu, Pei-Chieh;Huang, Li-Min;Chang, Luan-Yin
通讯作者：
Chang, Luan-Yin
人类肠道病毒 71 亚基因型 B3 在小鼠感染中缺乏柯萨奇病毒 A16 样神经毒力。
影响因子：
4.8
作者：
Chan YF;AbuBakar S
通讯作者：
AbuBakar S
影响因子：
4.4
作者：
Sarkis, Phuong Thi Nguyen;Ying, Songcheng;Yu, Xiao-Fang
通讯作者：
Yu, Xiao-Fang
影响因子：
4.2
作者：
Li Y;Zhu R;Qian Y;Deng J;Sun Y;Liu L;Wang F;Zhao L
通讯作者：
Zhao L