Muscle Fiber Population and Biochemical Properties of Whole Body Muscles in Thoroughbred Horses
Muscle Fiber Population and Biochemical Properties of Whole Body Muscles in Thoroughbred Horses
复制标题
DOI：
10.1002/ar.20961
复制
发表时间：
2009-10-01
影响因子：
2
通讯作者：
Miyata, Hirofumi
中科院分区：
文献类型：
--
作者：
Kawai, Minako;Minami, Yoshio;Miyata, Hirofumi
我们研究了肌肉纤维人口和骨骼肌的代谢特性，从整个身体的纯种马。尸检样本取自46个地点，在6个纯种马年龄在3至6岁。在用针对每种肌球蛋白重链同种型的单克隆抗体染色的肌纤维上确定纤维类型群体，并通过荧光定量法确定代谢酶活性。组织化学分析表明，大多数肌肉具有高百分比的Ha型纤维。就马体几个部位的肌肉特征而言，前肢肌肉的IIa型纤维百分比较高，IIx型纤维百分比显著低于后肢肌肉。在胸部和躯干部分的肌纤维类型的人口是相似的，在后肢部分。生化分析表明，高琥珀酸脱氢酶活性的疾病相关的肌肉和高磷酸果糖激酶活性的后肢。我们认为，纯种赛马中较高的Ha型纤维百分比归因于训练效果。为了进一步考虑身体每个部位的生理意义，需要运动期间每种肌纤维类型的招募模式的数据。本研究将肌纤维的特性与募集相结合。这些数据将为纯种马的生理和病理研究提供基础信息。Anat Rec，292：1663-1669，2009. (C)2009威利-利斯公司
We examine the muscle fiber population and metabolic properties of skeletal muscles from the whole body in Thoroughbred horses. Postmortem samples were taken from 46 sites in six Thoroughbred horses aged between 3 and 6 years. Fiber type population was determined on muscle fibers stained with monoclonal antibody to each myosin heavy chain isoform and metabolic enzyme activities were determined spectrophotometrically. Histochemical analysis demonstrated that most of the muscles had a high percentage of Type Ha fibers. In terms of the muscle characteristic in several parts of the horse body, the forelimb muscles had a higher percentage of Type IIa fiber and a significantly lower percentage of Type IIx fiber than the hindlimb muscles. The muscle fiber type populations in the thoracic and trunk portion were similar to those in the hindlimb portion. Biochemical analysis indicated high succinate dehydrogenase activity ill respiratory-related muscle and high phosphofructokinase activity in hindlimbs. We suggested that the higher percentage of Type Ha fibers in Thoroughbred racehorses is attributed to training effects. To consider further the physiological significance of each part of the body, data for the recruitment pattern of each muscle fiber type during exercise are needed. The muscle fiber properties in this study combined with the recruitment. data would provide fundamental information for physiological and pathological studies in Thoroughbred horses. Anat Rec, 292:1663-1669, 2009. (C) 2009 Wiley-Liss, Inc.