Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines
Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines
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DOI：
10.1016/j.ccr.2006.03.030
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发表时间：
2006-05-01
期刊：
影响因子：
50.3
通讯作者：
Fine, Howard A.
中科院分区：
文献类型：
--
作者：
Lee, Jeongwu;Kotliarova, Svetlana;Fine, Howard A.
肿瘤干细胞（TSCs）的概念为理解肿瘤生物学提供了一个新的范例，尽管目前尚不清楚TSCs是否会被证明是比传统癌细胞系更强大的模型。我们证明了显着的表型和基因型差异之间的主要人类肿瘤衍生的肿瘤干细胞和它们匹配的胶质瘤细胞系。与匹配的传统生长的肿瘤细胞系不同，直接来源于原发性胶质母细胞瘤的TSC与正常神经干细胞具有广泛的相似性，并概括了人类胶质母细胞瘤的基因型、基因表达模式和体内生物学。这些发现表明，与许多常用的癌细胞系相比，TSC可能是一种更可靠的模型，用于了解原发性人类肿瘤的生物学。
The concept of tumor stem cells (TSCs) provides a new paradigm for understanding tumor biology, although it remains unclear whether TSCs will prove to be a more robust model than traditional cancer cell lines. We demonstrate marked phenotypic and genotypic differences between primary human tumor-derived TSCs and their matched glioma cell lines. Unlike the matched, traditionally grown tumor cell lines, TSCs derived directly from primary glioblastomas harbor extensive similarities to normal neural stem cells and recapitulate the genotype, gene expression patterns, and in vivo biology of human glioblastomas. These findings suggest that TSCs may be a more reliable model than many commonly utilized cancer cell lines for understanding the biology of primary human tumors.