Postsynaptic activation of calcium/calmodulin-dependent protein kinase ii promotes coordinated pre- and postsynaptic maturation of drosophila neuromuscular junctions 1 1 These two authors contributed equally to this work.
Postsynaptic activation of calcium/calmodulin-dependent protein kinase ii promotes coordinated pre- and postsynaptic maturation of drosophila neuromuscular junctions 1 1 These two authors contributed equally to this work.
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DOI：
10.1016/s0306-4522(02)00923-5
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发表时间：
2003-03
期刊：
影响因子：
3.3
通讯作者：
H. Kazama;T. Morimoto-Tanifuji;A. Nose
中科院分区：
文献类型：
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作者：
H. Kazama;T. Morimoto-Tanifuji;A. Nose
神经元与其靶细胞之间的相互作用对于突触的发育至关重要。为了阐明靶细胞在突触发生中的作用，突触后钙/钙调素依赖性蛋白激酶II（CaMKII）的活性被操纵在一个马赛克的方式和其特定的效果进行了检查，在发展中的果蝇神经肌肉接头。我们发现，突触后表达的组成性活性CaMKII增强兴奋性突触电流（ESCs）的幅度和微型ESCs的频率。它还促进突触前和突触后特化的形态成熟，这可能是突触活动增强的基础。突触后细胞中CaMK Ⅱ抑制肽的表达部分影响了突触的成熟。这些结果表明两个显着的功能突触后CaMKII突触前特性和协调调节突触前和突触后成熟的突触发生-逆行调制。
The interaction between a neuron and its target cell(s) is essential for the development of synapses. To elucidate the role of target cells in synaptogenesis, the activity of postsynaptic calcium/calmodulin-dependent protein kinase II (CaMKII) was manipulated in a mosaic manner and its specific effect was examined at the developing Drosophila neuromuscular junction. We found that postsynaptic expression of constitutively active CaMKII augmented the amplitude of excitatory synaptic currents (ESCs) and the frequency of miniature ESCs. It also promoted morphological maturation of presynaptic as well as postsynaptic specializations, which presumably underlie the enhancement of synaptic activities. Expression of an inhibitory peptide of CaMKII in the postsynaptic cell partially affected the synaptic maturation. These results suggest two significant functions of postsynaptic CaMKII in synaptogenesis–retrograde modulation of presynaptic properties and coordinated regulation of pre- and postsynaptic maturation.