Characterisation of a New Family of Carboxyl Esterases with an OsmC Domain.
Characterisation of a New Family of Carboxyl Esterases with an OsmC Domain.
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DOI：
10.1371/journal.pone.0166128
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Rosser SJ
中科院分区：
文献类型：
--
作者：
Jensen MV;Horsfall LE;Wardrope C;Togneri PD;Marles-Wright J;Rosser SJ
丝氨酸酯酶家族中的蛋白质广泛分布于细菌门中，并表现出针对一系列生物产生和化学合成的酯的活性。最近鉴定了一种来自嗜冷细菌 Pseudoalteromonas arctica 的丝氨酸酯酶，其 C 端具有 OsmC 样结构域；在这里，我们报告了进一步假定的具有 OsmC 样结构域的酯酶的鉴定和表征，这些酯酶构成了一个新的酯酶家族，该家族存在于来自不同环境生态位的多种细菌物种中。所有这些蛋白质都含有丝氨酸酯酶共有的 Ser-Asp-His 基序和高度保守的五肽亲核肘基序。我们在大肠杆菌中异源产生这些蛋白质，并证明了它们针对一系列酯酶底物的活性。其中两种酯酶的活性比该家族其他成员高出两个数量级以上，并且在很宽的温度范围内都有活性。我们确定了来自Rhodothermus marinus的蛋白质的酯酶结构域的晶体结构，并表明它符合经典的α/β水解酶折叠，具有延伸的“盖子”区域，该区域封闭了晶体中蛋白质的活性位点。酯酶家族特征成员的扩展以及在较宽温度范围内的活性证明可用于生物技术应用，例如制药、洗涤剂、生物修复和乳制品行业。
Proteins in the serine esterase family are widely distributed in bacterial phyla and display activity against a range of biologically produced and chemically synthesized esters. A serine esterase from the psychrophilic bacterium Pseudoalteromonas arctica with a C-terminal OsmC-like domain was recently characterized; here we report on the identification and characterization of further putative esterases with OsmC-like domains constituting a new esterase family that is found in a variety of bacterial species from different environmental niches. All of these proteins contained the Ser-Asp-His motif common to serine esterases and a highly conserved pentapeptide nucleophilic elbow motif. We produced these proteins heterologously in Escherichia coli and demonstrated their activity against a range of esterase substrates. Two of the esterases characterized have activity of over two orders of magnitude higher than other members of the family, and are active over a wide temperature range. We determined the crystal structure of the esterase domain of the protein from Rhodothermus marinus and show that it conforms to the classical α/β hydrolase fold with an extended ‘lid’ region, which occludes the active site of the protein in the crystal. The expansion of characterized members of the esterase family and demonstration of activity over a wide-range of temperatures could be of use in biotechnological applications such as the pharmaceutical, detergent, bioremediation and dairy industries.
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DOI：
10.1107/s090744491003982x
发表时间：
2011-04
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Evans PR
通讯作者：
Evans PR
影响因子：
5.6
作者：
Bertrand, T.;Auge, F.;Mathieu, M.
通讯作者：
Mathieu, M.
DOI：
10.1039/jr9310001456
发表时间：
1931-01-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY
影响因子：
--
作者：
Britton, HTS;Robinson, RA
通讯作者：
Robinson, RA
影响因子：
2.9
作者：
Hess, Matthias;Katzer, Moritz;Antranikian, Garabed
通讯作者：
Antranikian, Garabed
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH