DISTINCT PHYSIOLOGICAL ROLES OF ANIMAL SUCCINATE THIOKINASES - ASSOCIATION OF GUANINE NUCLEOTIDE-LINKED SUCCINATE THIOKINASE WITH KETONE-BODY UTILIZATION
DISTINCT PHYSIOLOGICAL ROLES OF ANIMAL SUCCINATE THIOKINASES - ASSOCIATION OF GUANINE NUCLEOTIDE-LINKED SUCCINATE THIOKINASE WITH KETONE-BODY UTILIZATION
复制标题
DOI：
10.1016/0014-5793(86)80900-0
复制
发表时间：
1986-09-15
期刊：
影响因子：
3.5
通讯作者：
WEITZMAN, PDJ
中科院分区：
文献类型：
--
作者：
JENKINS, TM;WEITZMAN, PDJ
最近有两种不同的琥珀酸硫激酶被证明存在于动物组织中，一种是鸟嘌呤核苷酸的特异性酶，另一种是腺嘌呤核苷酸的特异性酶。通过比较正常大鼠和糖尿病大鼠肝脏和大脑中这两种酶的水平，研究了它们的生理作用。糖尿病患者脑鸟嘌呤核苷酸连接的琥珀酸硫激酶水平的显著升高与酮体利用的增强相一致，因此与乙酰乙酸激活所需的琥珀酰辅酶A的增加相关。结合报道的线粒体ATP/ADP值和GTP/GDP比值，结果被解释为表明腺嘌呤核苷酸连接酶作为柠檬酸添加循环的一个组成部分，而鸟嘌呤核苷酸连接酶在相反的代谢方向上发挥作用，从琥珀酸盐中产生琥珀酰辅酶a。
Two distinct succinate thiokinases have recently been shown to exist in animal tissues, one specific for guanine nucleotide and the other for adenine nucleotide. Their physiological roles have here been investigated by comparing the levels of the two enzymes in liver and brain of normal and diabetic rats. A marked rise in the level of brain guanine nucleotide‐linked succinate thiokinase in the diabetic condition is consistent with an enhanced utilization of ketone bodies and hence with the associated elevated demand for succinyl‐CoA for the activation of acetoacetate. Taken together with the reported mitochondrial values of the ATP/ADP and GTP/GDP ratios, the results are interpreted to indicate that the adenine nucleotide‐linked enzyme functions as a component of the citric add cycle whereas the guanine nucleotide‐linked enzyme functions in the opposite metabolic direction to produce succinyl‐CoA from succinate.