Functional Expression of Heterologous Proteins in Saccharomyces cerevisiae
Functional Expression of Heterologous Proteins in Saccharomyces cerevisiae
复制标题
酿酒酵母中异源蛋白的功能表达
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
G. Bitter
中科院分区：
文献类型：
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作者：
S. Thukral;K. Chang;G. Bitter
真核生物中许多细胞过程的分子机制在酵母和哺乳动物细胞之间是保守的。由于易于微生物操作和遗传表征，酿酒酵母通常用作研究来自其他真核细胞类型的蛋白质功能的替代宿主。为在酵母中高水平生产异源蛋白而开发的许多载体系统可用于本研究，但在酵母中成功表达具有生物活性的哺乳动物蛋白需要额外的考虑。本文介绍了现有的表达载体和蛋白分选系统，并讨论了在S.啤酒。
Abstract Molecular mechanisms of numerous cellular processes in eukaryotes are conserved between yeast and mammalian cells. Because of the ease of microbiological manipulation and genetic characterization, Saccharomyces cerevisiae is commonly used as a surrogate host for studying the function of proteins from other eukaryotic cell types. Numerous vector systems developed for high-level production of heterologous proteins in yeast are available for use in this research, but the successful expression of a biologically active mammalian protein in yeast requires additional considerations. In this paper, we describe the available expression vectors and protein sorting systems, and discuss appropriate strategies for expression of functional mammalian proteins in S. cerevisiae.