African Non-Human Primates Host Diverse Enteroviruses.
African Non-Human Primates Host Diverse Enteroviruses.
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DOI：
10.1371/journal.pone.0169067
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Leroy E
中科院分区：
文献类型：
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作者：
Mombo IM;Lukashev AN;Bleicker T;Brünink S;Berthet N;Maganga GD;Durand P;Arnathau C;Boundenga L;Ngoubangoye B;Boué V;Liégeois F;Ollomo B;Prugnolle F;Drexler JF;Drosten C;Renaud F;Rougeron V;Leroy E
肠道病毒（EV）属于小核糖核酸病毒科（Picornaviridae），并且引起哺乳动物（包括人类和非人类灵长类动物（NHP））中的轻度至重度疾病。猿猴EV最早于20世纪50年代在旧世界猴子中发现，最近在喀麦隆的野生黑猩猩，大猩猩和山鸡中发现。在本研究中，我们在加蓬的四个地点收集了600份野生猿和猴的粪便样本，通过PCR筛选EV。共有32个样本对EV呈阳性（25个来自山海关，7个来自黑猩猩，没有来自大猩猩）。VP 1和VP 2基因的系统发育分析表明，在黑猩猩中鉴定的EV是两种人类EV的成员，EV-A和EV-B，而在山鸡中鉴定的EV是人类EV的成员，EV-B和猿猴EV的成员。在黑猩猩中鉴定的两种新的肠道病毒类型，EV-B112和EV-B113，表明这些NHP可能是新EV类型的潜在来源。先前在人类中发现的EV-B107和EV 90的鉴定表明跨物种转移。此外，在山鸡中鉴定出黑猩猩来源的EV 110证明了该EV的广泛宿主范围。对NHP中EV的进一步研究将有助于了解新类型或变体的出现，并评估人类以及NHP人群跨物种传播的真实的风险。
Enteroviruses (EVs) belong to the family Picornaviridae and are responsible for mild to severe diseases in mammals including humans and non-human primates (NHP). Simian EVs were first discovered in the 1950s in the Old World Monkeys and recently in wild chimpanzee, gorilla and mandrill in Cameroon. In the present study, we screened by PCR EVs in 600 fecal samples of wild apes and monkeys that were collected at four sites in Gabon. A total of 32 samples were positive for EVs (25 from mandrills, 7 from chimpanzees, none from gorillas). The phylogenetic analysis of VP1 and VP2 genes showed that EVs identified in chimpanzees were members of two human EV species, EV-A and EV-B, and those identified in mandrills were members of the human species EV-B and the simian species EV-J. The identification of two novel enterovirus types, EV-B112 in a chimpanzee and EV-B113 in a mandrill, suggests these NHPs could be potential sources of new EV types. The identification of EV-B107 and EV90 that were previously found in humans indicates cross-species transfers. Also the identification of chimpanzee-derived EV110 in a mandrill demonstrated a wide host range of this EV. Further research of EVs in NHPs would help understanding emergence of new types or variants, and evaluating the real risk of cross-species transmission for humans as well for NHPs populations.