High-value utilization of the waste hydrolysate of Dioscorea zingiberensis for docosahexaenoic acid production in Schizochytrium sp.
High-value utilization of the waste hydrolysate of Dioscorea zingiberensis for docosahexaenoic acid production in Schizochytrium sp.
复制标题
薯蓣废水解液在裂殖壶菌中高值化生产二十二碳六烯酸。
DOI：
10.1016/j.biortech.2021.125305
复制
发表时间：
2021-05-24
影响因子：
11.4
通讯作者：
Yu, Longjiang
中科院分区：
文献类型：
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作者：
Bao, Zhendong;Zhu, Yuanmin;Yu, Longjiang
薯蓣块茎(SSLD)的淀粉糖化液是一种富含葡萄糖的农工业废物。在此，SSLD 被用作裂殖壶菌中二十二碳六烯酸（DHA）生物合成的新型潜在碳源。实现废物的回收利用和高值化利用。成分分析表明，SSLD含有丰富的营养成分，如葡萄糖、氨基酸、酚类和黄酮类化合物。当SSLD中总糖浓度优化为90 g/L时，生物量和DHA产量分别达到44.85和6.60 g/L，比纯葡萄糖培养条件提高了32.1%和36.92%。发酵特性和基因表达分析表明，SSLD能够显着提高细胞抗氧化能力，有利于清除细胞内活性氧，增加裂殖壶菌抗氧化酶基因的表达。因此，SSLD是裂殖壶菌生产DHA的有效且经济的碳源。
The starch saccharification liquid of Dioscorea zingiberensis tubers (SSLD) is a glucose-rich agro-industrial waste. Herein, SSLD was used as a novel potential carbon source for the biosynthesis of docosahexaenoic acid (DHA) in Schizochytrium sp. to achieve waste recycling and high-value utilization. Component analysis showed that SSLD contains abundant nutrients, such as glucose, amino acids, phenolics and flavonoids. When the total sugar concentration in SSLD was optimized to 90 g/L, the biomass and DHA yield reached 44.85 and 6.60 g/L, respectively, which were 32.1% and 36.92% higher than that at pure glucose culture condition. Fermentation characteristics and gene expression analysis showed that SSLD could remarkably improve cell antioxidant capacity, which is beneficial to scavenge intracellular reactive oxygen species and increase the gene expression of antioxidant enzymes in Schizochytrium sp. Hence, SSLD is an effective and economic carbon source for DHA production in Schizochytrium sp.