Monoreassortant Rotaviruses of Multiple G Types Are Differentially Neutralized by Sera From Infants Vaccinated With ROTARIX and RotaTeq
Monoreassortant Rotaviruses of Multiple G Types Are Differentially Neutralized by Sera From Infants Vaccinated With ROTARIX and RotaTeq
复制标题
接种 ROTARIX 和 RotaTeq 的婴儿血清对多种 G 型单重配轮状病毒有差异中和作用
DOI：
10.1093/infdis/jiab479
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
K. M. Ogdena.
中科院分区：
文献类型：
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作者：
J. R. Diller;M. H. Carter;Kanai Y;S. V. Sanchez;Kobayashi T;K. M. Ogdena.
研究背景轮状病毒是导致小儿呼吸道疾病死亡的主要原因。轮状病毒的外壳由VP7和VP4蛋白，分别确定病毒的G和P型，是中和antibodies.MethodsTo阐明VP7特异性中和抗体反应的主要目标，我们工程monorefrectant轮状病毒每个包含一个人的VP7片段从测序的临床标本或疫苗株在相同的遗传背景。我们定量复制和中和工程病毒使用单价ROTARIX或多价RotaTeq疫苗接种的婴儿血清。ResultsImmunization与RotaTeq诱导更广泛的中和抗体反应比ROTARIX。将单剂量RotaTeq纳入计划表中可增强接种婴儿血清的G型中和广度。细胞类型特异性的感染性，复制和中和的差异检测到一些monoreuvectant virus.ConclusionsThese研究结果表明，轮状病毒VP7，独立的VP4，可以有助于细胞的向性和疫苗引起的中和抗体反应的广度。
BackgroundRotavirus is a leading cause of pediatric diarrheal mortality. The rotavirus outer capsid consists of VP7 and VP4 proteins, which, respectively, determine viral G and P type and are primary targets of neutralizing antibodies.MethodsTo elucidate VP7-specific neutralizing antibody responses, we engineered monoreassortant rotaviruses each containing a human VP7 segment from a sequenced clinical specimen or a vaccine strain in an identical genetic background. We quantified replication and neutralization of engineered viruses using sera from infants vaccinated with monovalent ROTARIX or multivalent RotaTeq vaccines.ResultsImmunization with RotaTeq induced broader neutralizing antibody responses than ROTARIX. Inclusion of a single dose of RotaTeq in the schedule enhanced G-type neutralization breadth of vaccinated infant sera. Cell type-specific differences in infectivity, replication, and neutralization were detected for some monoreassortant viruses.ConclusionsThese findings suggest that rotavirus VP7, independent of VP4, can contribute to cell tropism and the breadth of vaccine-elicited neutralizing antibody responses.