Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa
Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa
复制标题
DOI：
10.1186/s12876-019-0986-0
复制
发表时间：
2019-05-14
影响因子：
2.4
通讯作者：
Njom, Henry
中科院分区：
文献类型：
--
作者：
Idowu, Ayodeji;Mzukwa, Asisipho;Njom, Henry
背景幽门螺杆菌的全球患病率接近50%，发达国家的患病率在20%至40%之间，非洲和世界其他发展中国家的患病率高达90%。幽门螺杆菌相关疾病的发展是由许多毒力因素决定的。本研究旨在确定幽门螺杆菌感染的患病率和毒力基因（cagA， dupA和vacA）；病人胃十二指肠疾病与毒力因素的关系。方法对患者进行胃活检并进行培养，从培养的分离物和活检组织中提取DNA进行PCR检测，然后按标准实验室程序对样本进行调查。通过问卷调查获得相关危险因素的数据。结果444例受试者中，培养法检出幽门螺旋杆菌115例（25.9%），直接PCR法检出幽门螺旋杆菌217例（48.9%）。PCR检出培养阳性患者98例（85.2%），总患病率为52.7%（234/444）。胃脘炎患者幽门螺杆菌分离率最高，为76.9%（180/234）。CagA毒力基因占62% (145/234)，dupA毒力基因占53.4% (125/234)，vacA毒力基因占90.6%（212/234）。VacA基因型s1m1最常见[56.4%(132)]，其次是s2m2[11.5%(27)]、s2m1[10.3%(24)]和s1m2[9.4%(22)]。研究发现，vacA s1与消化性溃疡疾病、vacA s1/m2与胃糜烂有显著相关性(P
BackgroundThe global prevalence of H. pylori approaches 50%, with prevalence rates between 20 and 40% in developed countries and up to 90% in Africa and other developing nations of the world. Development of H. pylori-associated diseases is determined by a number of virulence factors. This study aimed at determining the prevalence of H. pylori infections and virulence genes (cagA, dupA, and vacA); the relationship between virulence factors and gastroduodenal diseases among patients.MethodsGastric biopsies were obtained from patients and cultured, DNA was extracted from cultured isolates andbiopsies for PCR assay after which samples were investigated using standard laboratory procedures. Data of associated risk factors were obtained with the aid of questionnaires.ResultsOf the 444 participants, H. pylori was detected in 115 (25.9%) from culture analysis and 217 (48.9%) by direct PCR method. Ninety-eight (85.2%) of the culture-positive patients were also detected by PCR giving an overall prevalence of 52.7% (234/444). The highest number of H. pylori isolates 76.9% (180/234) was obtained from patients suffering from pangastritis. The CagA virulence gene was found in 62% (145/234), dupA in 53.4% (125/234) and vacA in 90.6% (212/234). VacA genotype s1m1 was the most prevalent [56.4% (132)] followed by s2m2 [11.5% (27)], s2m1 [10.3% (24)] and [s1m2 9.4% (22)]. There was a significant association observed in vacA s1 and peptic ulcer disease, as well as vacA s1/m2 and gastric erosion (P