Secretion of ions and pharmacological responsiveness in the mouse paw sweat gland.
Secretion of ions and pharmacological responsiveness in the mouse paw sweat gland.
复制标题
小鼠爪汗腺中离子的分泌和药理反应。
DOI：
10.1042/cs0860133
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Sato,F
中科院分区：
文献类型：
--
作者：
Sato,K;Cavallin,S;Sato,KT;Sato,F
1.本文描述了小鼠外泌汗腺的一些基本功能特征，以便将来与转基因小鼠进行比较.小鼠汗液分泌卷曲对乙酰甲胆碱有反应，如先前报道的，与大鼠爪汗腺一样，产生富含K+（> 120 mmol/l）、缺乏Na+（< 70 mmol/l）的初级流体。乙酰甲胆碱诱导的出汗率随着年龄的增长而增加，与生命前6周内汗腺的生长平行。对环腺苷酸升高剂（如异丙肾上腺素或毛喉素）的出汗反应在1周龄时高达乙酰甲胆碱诱导的出汗率的40%，但到6周龄时福尔斯下降至10%，尽管激动剂诱导的环腺苷酸组织累积（以每微克蛋白质为基础表示）在同一时期随年龄增加三倍。在体外超融合的成年小鼠分泌卷曲中，还观察到对乙酰甲胆碱的反应有明显的K+外流，并且观察到对环AMP升高激动剂的反应有小的K+外流。由于汗液分泌通常与K+通道或Cl−通道或两者的激活有关，并且由于出汗是对环AMP升高激动剂的反应，我们推测，环AMP激活的Cl−通道（囊性纤维化跨膜传导调节器的小鼠版本）也可能出现在小鼠汗腺中，但它们的表达程度可能受到小鼠年龄的影响。
1. Some of the basic functional features of the mouse paw eccrine sweat gland were delineated to allow comparison with those of transgenic mice in the future.2. The mouse sweat secretory coil responds to methacholine, elaborating a K+-rich (> 120 mmol/l), Na+-poor (< 70 mmol/l) primary fluid as does the rat paw sweat gland, as previously reported. The methacholine-induced sweat rate increases with age in parallel with the growth of the sweat gland over the first 6 weeks of life.3. The sweating response to cyclic AMP-elevating agents, such as isoprenaline or forskolin, is as much as 40% of the methacholine-induced sweat rate at 1 week of age, but falls to 10% by 6 weeks of age despite the fact that the agonist-induced tissue accumulation of cyclic AMP expressed on a per μg of protein basis triples with age over the same period.4. A marked K+outflux was also noted in response to methacholine and a small K+outflux was seen in response to cyclic AMP-elevating agonists in super-fused adult mouse secretory coilsin vitro.5. Since sweat secretion is usually associated with activation of either K+channels or Cl−channels or both, and since the sweating occurred in response to cyclic AMP-elevating agonists, we speculate that the cyclic AMP-activated Cl−channels (the mouse version of the cystic fibrosis transmembrane conductance regulator) may also occur in the mouse sweat gland, but that the degree of their expression may be influenced by the age of the mice.