Quantification of genetic diversity at allozyme loci
Quantification of genetic diversity at allozyme loci
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DOI：
10.1139/x96-195
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发表时间：
1997-03-01
影响因子：
2.2
通讯作者：
Hamrick, JL
中科院分区：
文献类型：
--
作者：
Berg, EE;Hamrick, JL
淀粉凝胶电泳法为分析植物种群和物种间的遗传变异提供了一种功能强大且经济高效的分析方法。等位酶基因座是典型的单基因性状，独立配对，共显性。在10-20个多态基因座上调查的30-50个个体的样本大小将充分描述种群中的遗传多样性，并表明遗传多样性在种群之间的分配方式。应报告的参数是多态座位百分比(P)、每个多态座位的平均等位基因数(AP)、期望杂合度(遗传多样性，H-e)、Nei‘s G(ST)、遗传同一性(I)和遗传距离(D)。给出了实例，并对参数估计的统计检验进行了总结。
Starch gel electrophoresis provides a powerful and cost-effective assay of genetic variation in plant populations and species. Allozyme loci are typically single-gene traits that assort independently and are codominant. Sample sizes of 30-50 individuals surveyed at 10-20 polymorphic loci will adequately characterize the genetic diversity in a population and indicate how genetic diversity is partitioned among populations. Parameters that should be reported are the percentage of polymorphic loci (P), mean number of alleles per polymorphic loci (AP), expected heterozygosity (genetic diversity, H-e), Nei's G(ST), genetic identity (I), and genetic distance (D). Examples are provided, and statistical tests of parameter estimates are summarized.