Metagenomes and metatranscriptomes from the L4 long-term coastal monitoring station in the Western English Channel.
Metagenomes and metatranscriptomes from the L4 long-term coastal monitoring station in the Western English Channel.
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DOI：
10.4056/sigs.1202536
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发表时间：
2010-10-27
影响因子：
--
通讯作者：
Field D
中科院分区：
文献类型：
--
作者：
Gilbert JA;Meyer F;Schriml L;Joint IR;Mühling M;Field D
超基因组数据和超转录组数据采集自西海峡天文台长期海岸-海洋监测站L4采样站（50.2518 N, 4.2089 W）的表层水（0-2m）。我们之前从该地区生成了一个为期六年的16S rRNA V6数据时间序列，该数据显示了细菌群落的强劲季节性结构，其多样性与白昼长度相关。在这里，我们描述这些宏基因组和亚转录组的特征。我们在2008年采样的8个时间点中生成了8个元基因组（450万个序列，1.9 Gbp，平均读长350 bp）和7个元转录组（392,632个推测mrna衍生序列，159 Mbp，平均读长272 bp）。这些时间点代表三个季节（冬季、春季和夏季），包括白天和夜晚的样本。这些数据表明了遗传潜力和现状之间的主要差异，即基因组遵循一般的季节性趋势，但随着时间的推移，功能潜力的变化却惊人地小；转录本的结构更倾向于在白天和黑夜之间发生变化。
Both metagenomic data and metatranscriptomic data were collected from surface water (0-2m) of the L4 sampling station (50.2518 N, 4.2089 W), which is part of the Western Channel Observatory long-term coastal-marine monitoring station. We previously generated from this area a six-year time series of 16S rRNA V6 data, which demonstrated robust seasonal structure for the bacterial community, with diversity correlated with day length. Here we describe the features of these metagenomes and metatranscriptomes. We generated 8 metagenomes (4.5 million sequences, 1.9 Gbp, average read-length 350 bp) and 7 metatranscriptomes (392,632 putative mRNA-derived sequences, 159 Mbp, average read-length 272 bp) for eight time-points sampled in 2008. These time points represent three seasons (winter, spring, and summer) and include both day and night samples. These data demonstrate the major differences between genetic potential and actuality, whereby genomes follow general seasonal trends yet with surprisingly little change in the functional potential over time; transcripts tended to be far more structured by changes occurring between day and night.
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影响因子：
9.8
作者：
Rusch DB;Halpern AL;Sutton G;Heidelberg KB;Williamson S;Yooseph S;Wu D;Eisen JA;Hoffman JM;Remington K;Beeson K;Tran B;Smith H;Baden-Tillson H;Stewart C;Thorpe J;Freeman J;Andrews-Pfannkoch C;Venter JE;Li K;Kravitz S;Heidelberg JF;Utterback T;Rogers YH;Falcón LI;Souza V;Bonilla-Rosso G;Eguiarte LE;Karl DM;Sathyendranath S;Platt T;Bermingham E;Gallardo V;Tamayo-Castillo G;Ferrari MR;Strausberg RL;Nealson K;Friedman R;Frazier M;Venter JC
通讯作者：
Venter JC
影响因子：
3
作者：
Meyer, F.;Paarmann, D.;Edwards, R. A.
通讯作者：
Edwards, R. A.
影响因子：
3
作者：
Niu B;Fu L;Sun S;Li W
通讯作者：
Li W
影响因子：
5.1
作者：
Gilbert, Jack A.;Field, Dawn;Joint, Ian
通讯作者：
Joint, Ian
影响因子：
46.9
作者：
Field, Dawn;Garrity, George;Wipat, Anil
通讯作者：
Wipat, Anil