Novel insights into host-pathogen interactions of large yellow croakers (Larimichthys crocea) and pathogenic bacterium Pseudomonas plecoglossicida using time-resolved dual RNA-seq of infected spleens
Novel insights into host-pathogen interactions of large yellow croakers (Larimichthys crocea) and pathogenic bacterium Pseudomonas plecoglossicida using time-resolved dual RNA-seq of infected spleens
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DOI：
10.24272/j.issn.2095-8137.2020.035
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发表时间：
2020-05-18
影响因子：
4.9
通讯作者：
Yan, Qing-Pi
中科院分区：
文献类型：
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作者：
Tang, Yi;Xin, Ge;Yan, Qing-Pi
宿主与病原体的相互作用非常复杂，涉及感染过程中基因表达的巨大动态变化。这些相互作用对于了解宿主的抗感染免疫以及病原体的发病机制至关重要。对于与动物宿主相互作用的细菌病原体，由于受感染组织中的病原体负载低，很难对受感染组织进行时间分辨双 RNA 测序。本研究建立了杀鱼假单胞菌感染大黄鱼的急性感染模型。受感染鱼的脾脏表现出典型的症状，在注射后两天（dpi）细菌载量达到最大。受感染脾脏的时间分辨双 RNA-seq 已成功应用于研究大黄鱼和 P. plecoglossicida 之间的宿主-病原体相互作用。对感染的大黄鱼的脾脏进行双重 RNA 测序，并将转录组数据与未感染的脾脏或体外培养的细菌的转录组数据进行比较。结果表明，病原体与宿主的相互作用受到高度动态调节，感染过程中宿主和病原体转录组也相应波动。在感染期间，涉及细胞因子细胞因子受体、Toll样受体信号传导和其他免疫相关通路的许多免疫基因的表达水平显着上调。此外，大黄鱼的代谢过程和氧气的利用受到 P. plecoglossicida 感染的强烈影响。 WGCNA结果表明，代谢过程与整个免疫过程密切相关。对于P. plecoglossicida，运动相关基因和鞭毛组装相关基因的表达水平显着上调。这项研究的结果可能有助于阐明大黄狗和 P. plecoglossicida 之间的相互作用。
Host-pathogen interactions are highly complex, involving large dynamic changes in gene expression during infection. These interactions are fundamental to understanding anti-infection immunity of hosts, as well as the pathogenesis of pathogens. For bacterial pathogens interacting with animal hosts, time-resolved dual RNA-seq of infected tissue is difficult to perform due to low pathogen load in infected tissue. In this study, an acute infection model of Larimichthys crocea infected by Pseudomonas plecoglossicida was established. The spleens of infected fish exhibited typical symptoms, with a maximum bacterial load at two days post-injection (dpi). Time-resolved dual RNA-seq of infected spleens was successfully applied to study host-pathogen interactions between L. crocea and P. plecoglossicida. The spleens of infected L. crocea were subjected to dual RNA-seq, and transcriptome data were compared with those of noninfected spleens or in vitro cultured bacteria. Results showed that pathogen-host interactions were highly dynamically regulated, with corresponding fluctuations in host and pathogen transcriptomes during infection. The expression levels of many immunogenes involved in cytokine-cytokine receptor, Toll-like receptor signaling, and other immune-related pathways were significantly up-regulated during the infection period. Furthermore, metabolic processes and the use of oxygen in L. crocea were strongly affected by P. plecoglossicida infection. The WGCNA results showed that the metabolic process was strongly related to the entire immune process. For P. plecoglossicida, the expression levels of motility-related genes and flagellum assembly-related genes were significantly up-regulated. The results of this study may help to elucidate the interactions between L. crocea and P. plecoglossicida.