Penicillium species endophytic in coffee plants and ochratoxin A production.
Penicillium species endophytic in coffee plants and ochratoxin A production.
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DOI：
10.3852/mycologia.98.1.31
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发表时间：
2006
期刊：
影响因子：
2.8
通讯作者：
F. Vega;Francisco J. Posada;S. Peterson;T. Gianfagna;F. Chaves
中科院分区：
文献类型：
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作者：
F. Vega;Francisco J. Posada;S. Peterson;T. Gianfagna;F. Chaves
在哥伦比亚、夏威夷和马里兰州的一个当地植物苗圃采集的阿拉比卡咖啡、congensis、C. dewevrei和C. liberica的组织进行了真菌内生菌的取样。表面无菌组织包括根，叶，茎和各种浆果部分被镀在酵母麦芽琼脂。从一组目视识别为青霉菌的分离株中提取DNA，对其内部转录间隔区和部分LSU-rDNA进行扩增和测序。与GenBank和未发表序列的DNA序列比较，发现存在11种已知青霉菌：P. breviccompacactum、P. brocae、P. cecidicola、P. citriinum、P. coffae、P. crustosum、P. janthinellum、P. olsonii、P. oxalicum、P. sclerotiorum和P. steckii，以及P. diversum和P. roseopurpureum附近可能未被描述的种。产赭曲霉毒素A的菌株只有4株，分别为短壳菌、壳菌、赭曲霉和草酸菌各1株。这些内生菌在咖啡树的生物学中所起的作用仍然是个谜。
Tissues from Coffea arabica, C. congensis, C. dewevrei and C. liberica collected in Colombia, Hawaii and at a local plant nursery in Maryland were sampled for the presence of fungal endophytes. Surface sterilized tissues including roots, leaves, stems and various berry parts were plated on yeast-malt agar. DNA was extracted from a set of isolates visually recognized as Penicillium, and the internal transcribed spacer region and partial LSU-rDNA was amplified and sequenced. Comparison of DNA sequences with GenBank and unpublished sequences revealed the presence of 11 known Penicillium species: P. brevicompactum, P. brocae, P. cecidicola, P. citrinum, P. coffeae, P. crustosum, P. janthinellum, P. olsonii, P. oxalicum, P. sclerotiorum and P. steckii as well as two possibly undescribed species near P. diversum and P. roseopurpureum. Ochratoxin A was produced by only four isolates, one isolate each of P. brevicompactum, P. crustosum, P. olsonii and P. oxalicum. The role these endophytes play in the biology of the coffee plant remains enigmatic.