miR-224-5p regulates the proliferation, migration and invasion of pancreatic mucinous cystadenocarcinoma by targeting PTEN
miR-224-5p regulates the proliferation, migration and invasion of pancreatic mucinous cystadenocarcinoma by targeting PTEN
复制标题
miR-224-5p通过靶向PTEN调控胰腺粘液性囊腺癌的增殖、迁移和侵袭
DOI：
10.3892/mmr.2021.11985
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发表时间：
2021-05-01
影响因子：
3.4
通讯作者：
Zhan, Xianbao
中科院分区：
文献类型：
--
作者：
Peng, Xiaobo;Guo, Chengtao;Zhan, Xianbao
胰腺粘液性囊腺癌是一种罕见的恶性肿瘤，相关研究较少。本研究旨在探讨microRNA（miR）-224-5p在胰腺MCC增殖、迁移和侵袭中的作用及其机制。逆转录-定量PCR检测miR-224- 5 p和PTEN基因的表达。采用MTT法、创伤愈合实验、Transwell实验和成瘤实验观察MCC 1细胞在体外和体内的增殖、迁移和侵袭能力。Western blot分析PTEN蛋白表达。通过荧光素酶试验评估并验证了miR-224- 5 p的靶基因。与正常组织和细胞相比，在MCC 1细胞中miR-224- 5 p表达显著升高，而PTEN表达降低。过表达miR-224- 5 p可促进MCC的增殖、迁移和侵袭，而敲低miR-224- 5 p可抑制MCC的增殖、迁移和侵袭。生物信息学分析和荧光素酶检测结果表明，PTEN是miR-224- 5 p的直接靶基因。miR-224- 5 p和PTEN之间的负相关性在体外和体内均得到证实。PTEN逆转miR-224- 5 p对MCC 1细胞增殖、迁移和侵袭的影响。据作者所知，本研究首次揭示了miR-224- 5 p在MCC中的高表达和致癌作用。miR-224- 5 p不仅调节胰腺MCC的增殖、迁移和侵袭，而且可能成为MCC潜在的治疗靶点。
Pancreatic mucinous cystadenocarcinoma (MCC) is a rare malignant tumor, with a limited number of studies. The present study aimed to investigate the function and mechanism of microRNA (miR)-224-5p on proliferation, migration and invasion of MCC of the pancreas. Reverse transcription-quantitative PCR was used to explorethe expression of miR-224-5p and the PTEN gene. MTT, wound healing, Transwell and tumorigenesis assays were conducted to investigate the proliferation, migration and invasion of MCC1 cells in vitro and in vivo. Western blot analysis was employed to test the protein expression of PTEN. The target gene of miR-224-5p was assessed and verified by luciferase assay. miR-224-5p expression was notably higher, while PTEN expression was lower, in MCC1 cells compared with normal tissues and cells. Overexpression of miR-224-5p promoted the proliferation, migration and invasion of MCC and knockdown of miR-224-5p inhibited these functions. Bioinformatics analysis and luciferase assay indicated that PTEN was the direct target gene of miR-224-5p. The negative correlation between miR-224-5p and PTEN was confirmed both in vitro and in vivo. PTEN reversed the effects of miR-224-5p on proliferation, migration and invasion of MCC1 cells. The present study revealed for the first time, to the best of the authors' knowledge, that miR-224-5p was highly expressed and served an oncogenic role in MCC. miR-224-5p not only regulated the proliferation, migration and invasion of pancreatic MCC but may also be a potential therapeutic target for MCC.