Association study highlights the influence of ELOVL fatty acid elongase 6 gene region on backfat fatty acid composition in Large White pig breed.
Association study highlights the influence of ELOVL fatty acid elongase 6 gene region on backfat fatty acid composition in Large White pig breed.
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DOI：
10.1017/s1751731118000484
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发表时间：
2018-03
期刊：
影响因子：
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通讯作者：
M. Zappaterra;R. Ros-Freixedes;J. Estany;R. Davoli
中科院分区：
文献类型：
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作者：
M. Zappaterra;R. Ros-Freixedes;J. Estany;R. Davoli
膳食脂肪酸（FA）组成对人体健康有重要影响。消费者对健康食品的要求越来越高，猪肉行业必须在不降低猪肉产品性能和技术特性的情况下做出回应。在生猪选择方案中加入胴体脂肪酸组成的遗传标记，将有助于在不饱和脂肪酸和饱和脂肪酸之间达到适当的平衡，以满足市场需求。为了寻找与猪背脂肪脂肪酸组成相关的基因组区域，利用Illumina porcinesnp60k对798头意大利大白猪进行了全基因组关联研究，发现与棕榈酸、棕榈油酸、油酸、中链和长链脂肪酸含量相关性最强的区域位于su scrofa染色体（SSC） 8区，位于119 ~ 122 Mb， ELOVL FA延长酶6基因在该区域被定位。棕榈酸、棕榈油酸、硬脂酸和油酸含量也被发现与SSC14相关，特别是在121至124 Mb的基因组区域，其中硬脂酰辅酶a去饱和酶Δ9基因所在。另一方面，与花生四烯果、花生四烯果、n-6和n-3脂肪酸背脂含量相关的基因组区域主要包含与膳食脂质和碳水化合物消化、吸收和利用有关的基因。据我们所知，这是首次在大型白猪身上进行的研究，确定了与背膘FA组成相关的标记和基因组区域。结果证实了之前在其他猪品种中发现的一些关联，并表明不同的代谢途径参与了必需和非必需脂肪酸的沉积模式。
Dietary fatty acid (FA) composition has an impact on human health. There is an increasing request from consumers for healthier food and pork industry must respond to it without worsening performance and the technological properties of pork products. The inclusion of genetic markers for carcass FA composition in pig selection schemes could be a useful tool to reach the right balance between unsaturated and saturated FAs to satisfy market demands. With the aim of finding genomic regions associated with porcine backfat FA composition, a genome-wide association study was performed on 798 Italian Large White pigs genotyped using Illumina PorcineSNP60 k. The strongest associations with backfat contents of palmitic, palmitoleic, oleic, medium-chain and long-chain FAs were found for the Sus scrofa chromosome (SSC) 8 region located at 119 to 122 Mb, where the gene ELOVL FA elongase 6 is mapped. Palmitic, palmitoleic, stearic and oleic acid contents were also found associated with SSC14, in particular with the genomic region at 121 to 124 Mb, where stearoyl-CoA desaturase Δ9 gene lies. On the other hand, the genomic regions associated with backfat contents of arachidic, arachidonic, n-6 and n-3 FAs showed to harbour mainly genes involved in dietary lipids and carbohydrates digestion, absorption and utilisation. To our knowledge, this is the first study performed in Large White pigs identifying markers and genomic regions associated with backfat FA composition. The results validate in Large White some associations previously detected in other pig breeds and indicate the involvement of distinct metabolic pathways in the deposition pattern of essential and non-essential FAs.