Comparative genomics to bridge Vicia faba with model and closely-related legume species: stability of QTLs for flowering and yield-related traits
Comparative genomics to bridge Vicia faba with model and closely-related legume species: stability of QTLs for flowering and yield-related traits
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DOI：
10.1007/s00122-012-1952-1
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发表时间：
2012-12-01
影响因子：
5.4
通讯作者：
Torres, A. M.
中科院分区：
文献类型：
--
作者：
Cruz-Izquierdo, S.;Avila, C. M.;Torres, A. M.
这项研究介绍了蚕豆增强MAP的开发。该图谱包含258个座位，大部分是基于基因的标记，分为16个连锁群，扩展1875 cM，平均标记间距离为7.26 cM。EST来源的标记与一些通过三体分离物理定位或先前归属于染色体的标记相结合，允许将8个连锁群(229个标记)分配到特定的染色体上。此外，这种方法为建立蚕豆染色体和近缘豆科植物染色体之间的全球同源性提供了锚点。首次利用该图谱对开花天数、开花长度、角果长度、每角粒数和每角果胚珠数这5个开花和生殖性状的QTL进行了定位和验证。在II、V和VI染色体上检测到12个QTL，这些QTL在第2、V和VI染色体上稳定存在。比较图谱表明，在其他5种豆科植物(紫花苜蓿、莲花、豌豆、羽扇豆、鹰嘴豆)中，控制开花性状天数的一个蚕豆基因组区域是保守的。控制蚕豆和莲花之间角果长度和每角粒数的QTL可能存在额外的共线共定位。新的遗传图谱为进一步研究蚕豆与相关豆科植物之间的基因转化开辟了道路，并为QTL分析和分子标记辅助选择等育种应用提供了有效的工具。
This study presents the development of an enhanced map in faba bean. The map contains 258 loci, mostly gene-based markers, organized in 16 linkage groups that expand 1,875 cM, with an average inter-marker distance of 7.26 cM. The combination of EST-derived markers with a number of markers physically located or previously ascribed to chromosomes by trisomic segregation, allowed the allocation of eight linkage groups (229 markers), to specific chromosomes. Moreover, this approach provided anchor points to establish a global homology among the faba bean chromosomes and those of closely-related legumes species. The map was used to identify and validate, for the first time, QTLs controlling five flowering and reproductive traits: days to flowering, flowering length, pod length, number of seeds per pod and number of ovules per pod. Twelve QTLs stable in the 2 years of evaluation were identified in chromosomes II, V and VI. Comparative mapping suggested the conservation of one of the faba bean genomic regions controlling the character days to flowering in other five legume species (Medicago, Lotus, pea, lupine, chickpea). Additional syntenic co-localizations of QTLs controlling pod length and number of seeds per pod between faba bean and Lotus japonicus are likely. The new genetic map opens the way for further translational studies between faba bean and related legume species, and provides an efficient tool for breeding applications such as QTL analysis and marker-assisted selection.