A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus
A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus
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雪松松针中 2R,3R-二氢杨梅素对金黄色葡萄球菌的膜破坏和 DNA 结合的双重抗菌机制
DOI：
10.1016/j.foodchem.2016.07.090
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发表时间：
2017-03-01
期刊：
影响因子：
8.8
通讯作者：
Gao, Hong
中科院分区：
文献类型：
--
作者：
Wu, Yanping;Bai, Jinrong;Gao, Hong
研究了2R，3R-二氢杨梅素(DMY)对金黄色葡萄球菌的抗菌活性及其作用机制。DMY对金黄色葡萄球菌的最低抑菌浓度为0.125 mg/ml，生长抑制实验也显示其对金黄色葡萄球菌有较强的抗菌活性。大量的核苷酸泄漏和流式细胞仪分析表明，DMY破坏了金黄色葡萄球菌的膜完整性。DMY处理的金黄色葡萄球菌细胞的形态变化和膜超极化进一步表明DMY破坏了细胞膜。同时，DMY可能与膜脂和膜蛋白相互作用，导致膜流动性显著降低，膜蛋白构象发生改变。此外，DMY可与金黄色葡萄球菌DNA以沟槽结合方式相互作用。总体而言，DMY通过破坏细胞膜和与细胞内DNA结合而达到杀菌活性，因此可以作为开发新型食品防腐剂的候选物质。(C)爱思唯尔有限公司出版的2016年。
The antibacterial activity and mechanism of 2R,3R-dihydromyricetin (DMY) against Staphylococcus aureus were investigated. The minimum inhibitory concentration of DMY against S. aureus was 0.125 mg/ml, and the growth inhibitory assay also revealed that DMY showed a potent antibacterial activity against S. aureus. Massive nucleotide leakage and flow cytometric analysis demonstrated that DMY disrupted the membrane integrity of S. aureus. Morphological changes and membrane hyperpolarization of S. aureus cells treated with DMY further suggested that DMY destroyed cell membrane. Meanwhile, DMY probably interacted with membrane lipids and proteins, causing a significant reduction in membrane fluidity and changes in conformation of membrane protein. Moreover, DMY could interact with S. aureus DNA through the groove binding mode. Overall, the results suggested that DMY could be applied as a candidate for the development of new food preservatives as it achieved bactericidal activity by damaging cell membrane and binding to intracellular DNA. (C) 2016 Published by Elsevier Ltd.