Calcium imaging of sleep-wake related neuronal activity in the dorsal pons.
Calcium imaging of sleep-wake related neuronal activity in the dorsal pons.
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DOI：
10.1038/ncomms10763
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发表时间：
2016-02-25
影响因子：
16.6
通讯作者：
Dan Y
中科院分区：
文献类型：
--
作者：
Cox J;Pinto L;Dan Y
长期以来，背侧脑桥一直与快速眼动（REM）睡眠的产生有关，但对潜在的回路机制仍然知之甚少。使用细胞类型特异性显微内窥镜Ca 2+成像和附近的背外侧被盖核，我们发现，许多多巴胺能神经元最活跃的REM睡眠（REM-最大），而大多数GABA能神经元最活跃的觉醒（最大）。此外，多巴胺能神经元的活动表现出内侧-外侧空间梯度，位于内侧的神经元在REM睡眠期间更有选择性地活跃。 做梦发生在快速眼动睡眠中，但产生它的神经机制还不清楚。考克斯及其同事发现，背侧脑桥中的谷氨酸能神经元在向REM睡眠过渡期间最活跃，而GABA能神经元在清醒状态下更活跃。
The dorsal pons has long been implicated in the generation of rapid eye movement (REM) sleep, but the underlying circuit mechanisms remain poorly understood. Using cell-type-specific microendoscopic Ca2+ imaging in and near the laterodorsal tegmental nucleus, we found that many glutamatergic neurons are maximally active during REM sleep (REM-max), while the majority of GABAergic neurons are maximally active during wakefulness (wake-max). Furthermore, the activity of glutamatergic neurons exhibits a medio-lateral spatial gradient, with medially located neurons more selectively active during REM sleep. Dreaming occurs in REM sleep, yet the neural mechanisms involved in generating it are not understood. Here Cox and colleagues show that glutamatergic neurons in the dorsal pons are activated most during transition to REM sleep while GABAergic neurons are more active during waking state.