A survey of Chinese pig farms and human healthcare isolates reveals separate human and animal MRSA populations-猫眼课题宝

DOI：

10.1101/2021.08.02.454852

发表时间：

2021-08

期刊：

bioRxiv

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

Geng Zou;M. Matuszewska;Ming Jia;Jianwei Zhou;X. Ba;J. Duan;Caishi Zhang;Jian Zhao;Meng-Ting Tao;Jingyan Fan;Xiangming Zhang;Wenping Jin;T. Cui;Xianyu Zeng;Ming Jia;Xiaojuan Qian;Chao Huang;Wen-Jian Zhuo;Z. Yao;Lijun Zhang;Shaowen Li;Lu Li;Qi Huang;Bin Wu;Huanchun Chen;A. Tucker;A. Grant;M. Holmes;R. Zhou

人们越来越担心，畜牧业中过度使用抗生素会增加人们的抗菌素耐药性负担。欧洲和北美的养殖动物，特别是猪，为人类中发现的家畜相关耐甲氧西林金黄色葡萄球菌（LA-MRSA，ST 398）提供了储存库。本研究旨在调查中国猪场MRSA对人类感染和携带的贡献。2017-2018年期间，从中国中部的55个猪场和4家医院（MRSA= 285，198; MSSA= 50，70）中分离出金黄色葡萄球菌。CC 9型MRSA占所有农场MRSA分离株的93%，而在医院中未发现。在3个农场（n = 23）和3家医院（n = 12）发现了ST 398分离株。本研究中的ST 398均不属于欧洲和北美常见的LA-MRSA的牲畜分支。医院的ST 398 MRSA分离株形成了一个进化枝，该进化枝与农场的ST 398 MRSA和MSSA分离株明显不同，并且都具有人免疫逃避簇基因，这些基因在所有猪场的ST 398分离株中均不存在。尽管在中国养猪场发现了高水平的MRSA，但我们没有发现它们扩散到人类的证据。然而，从人体样本中获得的ST 398 MRSA似乎是中国广泛分布的谱系的一部分。在中国出现的新的动物适应性ST 398谱系也应该引起警惕。重要性我们披露了这样一个事实，尽管中国医院和养猪场的高MRSA阳性率应该引起警惕，但它们可能是两个独立的问题。我们鉴定的新的CC 398进化枝强调了MRSA谱系的宿主适应是不断变化的。这些结果表明，继续监测MRSA在牲畜是必要的。我们发现猪场MRSA分离株具有独特的抗菌药物耐药基因，而医院MRSA分离株大多具有人类免疫逃避簇基因。这些特征可用于区分猪场相关的S.金黄色葡萄球菌临床实验室。自二零二零年起，中国实施减少牲畜使用抗菌剂的政策。我们的研究描述了2020年前华中地区养猪场和医院中MRSA种群的情况，这为未来评估政策效果的研究提供了潜在的机会。

There has been increasing concern that the overuse of antibiotics in livestock farming is contributing to the burden of antimicrobial resistance in people. Farmed animals in Europe and North America, particularly pigs, provide a reservoir for livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA, ST398) found in people. This study was designed to investigate the contribution of MRSA from Chinese pig farms to human infection and carriage.A collection of 603 S. aureus were isolated from 55 pig farms and 4 hospitals (MRSA= 285, 198; MSSA= 50, 70) in central China, a high pig farming density area, during 2017-2018. CC9 MRSA accounting for 93% of all farm MRSA isolates, while no was found in hospitals. ST398 isolates were found on three farms (n = 23) and three hospitals (n = 12). None of the ST398 from this study belong to the livestock clade of the LA-MRSA commonly found in Europe and North America. The hospital ST398 MRSA isolates formed a clade that was clearly separate from the farm ST398 MRSA and MSSA isolates, and all possessed human immune evasion cluster genes which were absent from all the pig farm ST398 isolates. Despite the presence of high levels of MRSA found on Chinese pig farms we found no evidence of them spilling over to the human population. Nevertheless, the ST398 MRSA obtained from human samples appear to be part of a widely distributed lineage in China. And the new animal adapted ST398 lineage that emerged in China should also be alarmed. Importance We disclosed the fact that although the high MRSA positive rate in Chinese hospitals and pig farms should be alarmed, they might be two separate issues. The new CC398 clades we identified highlight that the host adaption of the MRSA lineage is kept changing. These results suggest that continued surveillance of MRSA in livestock is necessary. We found that the pig farm MRSA isolates had unique antimicrobial resistance genes while most of the hospital MRSA isolates had human immune evasion cluster genes. These features could be used to distinguish the pig farm associated S. aureus in clinical laboratories. The policies of reducing antimicrobials use in livestock were implemented in China since 2020. Our study described the situation of MRSA populations in pig farms and hospitals in Central China before 2020, which provides a potential opportunity for future studies to evaluate the effects of the policies.

A survey of Chinese pig farms and human healthcare isolates reveals separate human and animal MRSA populations

文献摘要

相似文献