Visualization of Dynamic Conformational Switching of the G-Quadruplex in a DNA Nanostructure
Visualization of Dynamic Conformational Switching of the G-Quadruplex in a DNA Nanostructure
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DOI：
10.1021/ja1058907
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发表时间：
2010-11-24
影响因子：
15
通讯作者：
Sugiyama, Hiroshi
中科院分区：
文献类型：
--
作者：
Sannohe, Yuta;Endo, Masayuki;Sugiyama, Hiroshi
我们在此报告的实时观察G-四链体的形成，通过监测G-四链体诱导的全球变化的两个双链体纳入DNA纳米支架。引入的富含G的链在K+存在下形成链间（3 + 1）G-四链体结构，并且所形成的四链结构通过去除K而被破坏。这些构象变化是可视化的纳米支架在实时与快速扫描原子力显微镜。
We herein report the real-time observation of G-quadruplex formation by monitoring the G-quadruplex-induced global change of two duplexes incorporated in a DNA nanoscaffold. The introduced G-rich strands formed an interstrand (3 + 1) G-quadruplex structure in the presence of K+, and the formed four-stranded structure was disrupted by removal of K. These conformational changes were visualized in a nanoscaffold in real-time with fast-scanning atomic force microscopy.