DEVELOPMENT OF NADPH-PRODUCING PATHWAYS IN RAT-HEART
DEVELOPMENT OF NADPH-PRODUCING PATHWAYS IN RAT-HEART
复制标题
DOI：
10.1042/bj1860799
复制
发表时间：
1980-01-01
影响因子：
4.1
通讯作者：
MACHADO, A
中科院分区：
文献类型：
--
作者：
ANDRES, A;SATRUSTEGUI, J;MACHADO, A
研究了大鼠心脏发育过程中主要的NADPH产生酶(G-6-P脱氢酶、6-磷酸葡萄糖酸脱氢酶、细胞质和线粒体苹果酸酶、NADP+依赖的异柠檬酸脱氢酶)的行为，并与脑和肝脏的NADPH产生酶进行了比较。在整个发育过程中，属于磷酸戊糖途径的酶在心脏中的活性低于其他组织。心脏细胞质和线粒体苹果酸酶的诱导模式与在肝脏中发现的模式不同。从出生起，心脏线粒体酶就被缓慢地诱导。NADP+依赖的异柠檬酸脱氢酶在18天胎儿的所有组织中具有相似的活性。心脏线粒体NADP+依赖的异柠檬酸脱氢酶在成人中被大量诱导，其活性是肝脏的10倍。讨论了线粒体苹果酸酶和NADP+依赖的异柠檬酸脱氢酶的生理功能。
The behaviors of the principal NADPH-producing enzymes (G-6-P dehydrogenase, 6-phosphogluconate dehydrogenase, cytoplasmic and mitochondrial malic enzyme and NADP+-dependent isocitrate dehydrogenase) were studied during the development of rat heart and compared with those in brain and liver. Enzymes belonging to the pentose phosphate pathway exhibit lower activities in heart than in other tissues throughout development. The pattern of induction of heart cytoplasmic and mitochondrial malic enzymes does not parallel that found in liver. Heart mitochondrial enzyme is slowly induced from birth onwards. NADP+-dependent isocitrate dehydrogenase has similar activities in all tissues in 18-day fetuses. Heart mitochondrial NADP+-dependent isocitrate dehydrogenase is greatly induced in the adult, where it attains a 10-fold higher activity than in liver. Physiological functions of mitochondrial malic enzyme and NADP+-dependent isocitrate dehydrogenase are discussed.