Involvement of opsins in mammalian sperm thermotaxis.
Involvement of opsins in mammalian sperm thermotaxis.
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DOI：
10.1038/srep16146
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发表时间：
2015-11-05
影响因子：
4.6
通讯作者：
Eisenbach M
中科院分区：
文献类型：
--
作者：
Pérez-Cerezales S;Boryshpolets S;Afanzar O;Brandis A;Nevo R;Kiss V;Eisenbach M
哺乳动物精子趋热性的一个独特特征是极端的温度敏感性，表现为精子在游动其体长距离时对<0.0006 °C的温度变化的反应能力。赋予精子这种特殊敏感性的传感系统的身份尚不清楚。在这里，我们表明，哺乳动物精子的温度传感系统涉及视蛋白，已知是G-蛋白偶联受体，在视觉中作为光传感器。我们证明了分子，免疫学和功能的方法，视蛋白存在于人类和小鼠精子的特定位点，这取决于物种和视蛋白的类型，并参与精子的趋热性通过两个信号通路-磷脂酶C和环核苷酸途径。我们的研究结果表明，根据上下文和组织，哺乳动物视蛋白不仅作为光传感器，而且作为热敏元件。
A unique characteristic of mammalian sperm thermotaxis is extreme temperature sensitivity, manifested by the capacity of spermatozoa to respond to temperature changes of <0.0006 °C as they swim their body-length distance. The identity of the sensing system that confers this exceptional sensitivity on spermatozoa is not known. Here we show that the temperature-sensing system of mammalian spermatozoa involves opsins, known to be G-protein-coupled receptors that act as photosensors in vision. We demonstrate by molecular, immunological, and functional approaches that opsins are present in human and mouse spermatozoa at specific sites, which depend on the species and the opsin type, and that they are involved in sperm thermotaxis via two signalling pathways—the phospholipase C and the cyclic-nucleotide pathways. Our results suggest that, depending on the context and the tissue, mammalian opsins act not only as photosensors but also as thermosensors.