Expression of protein kinase C‐substrate mRNAs in the basal ganglia of adult and infant macaque monkeys
Expression of protein kinase C‐substrate mRNAs in the basal ganglia of adult and infant macaque monkeys
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DOI：
10.1002/cne.21119
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发表时间：
2006-12
影响因子：
2.5
通讯作者：
N. Higo;T. Oishi;A. Yamashita;Y. Murata;K. Matsuda;M. Hayashi
中科院分区：
文献类型：
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作者：
N. Higo;T. Oishi;A. Yamashita;Y. Murata;K. Matsuda;M. Hayashi
我们在猴子基底节区进行原位杂交组织化学，研究了三种蛋白激酶C底物（GAP‐43，MARCKS和neurogranin）的mRNA定位，它们的表达在神经突和突触的结构变化中起作用。在成年新纹状体中观察到GAP‐43 mRNA的弱杂交信号和marks和神经粒蛋白mRNA的强杂交信号。这三种mrna均在P物质阳性的直接通路神经元和脑啡肽阳性的间接通路神经元中表达。在伏隔核中，这三种mrna的杂交信号较新纹状体弱。新纹状体双标记原位杂交组织化学显示，GAP - 43和神经粒蛋白mrna在一部分MARCKS阳性神经元中表达。尽管在所有其他基底神经节区域（如苍白球、无名质、丘脑底核和黑质）均观察到MARCKS mRNA的强烈杂交信号，但GAP‐43 mRNA的强烈杂交信号仅限于无名质和黑质致密部。新纹状体和伏隔核以外的基底节区未见神经粒蛋白mRNA的表达。这些结果表明，在皮质-基底神经节回路的某些特定连接中，蛋白激酶C底物丰富。发育分析表明，该基因在婴儿壳核和伏隔核中的表达水平高于成人，而在尾状核中没有表达，表明尾状核的突触成熟早于壳核和伏隔核。[j]中华神经医学杂志，2006。©2006 Wiley‐Liss, Inc。
We performed in situ hybridization histochemistry on the monkey basal ganglia to investigate the mRNA localization of three protein kinase C substrates (GAP‐43, MARCKS, and neurogranin), of which expression plays a role in structural changes in neurites and synapses. Weak hybridization signals for GAP‐43 mRNA and intense signals for both MARCKS and neurogranin mRNAs were observed in the adult neostriatum. All three of the mRNAs were expressed in both substance P‐positive direct pathway neurons and enkephalin‐positive indirect pathway neurons. In the nucleus accumbens, the hybridization signals for the three mRNAs were weaker than those in the neostriatum. Double‐label in situ hybridization histochemistry in the neostriatum revealed that GAP‐43 and neurogranin mRNAs were expressed in a subset of MARCKS‐positive neurons. While intense hybridization signals for MARCKS mRNA were observed in all of the other basal ganglia regions such as the globus pallidus, substantia innominata, subthalamic nucleus, and substantia nigra, intense signals for GAP‐43 mRNA were restricted to the substantia innominata and substantia nigra pars compacta. No signal for neurogranin mRNA was observed in the basal ganglia regions outside the neostriatum and the nucleus accumbens. These results indicate that the protein kinase C substrates are abundant in some specific connections in cortico‐basal ganglia circuits. Developmental analysis showed that the expression level in the putamen and nucleus accumbens, but not in the caudate nucleus, was higher in the infant than in the adult, suggesting that synaptic maturation in the caudate nucleus occurs earlier than that in the putamen and nucleus accumbens. J. Comp. Neurol. 499:662–676, 2006. © 2006 Wiley‐Liss, Inc.