Heat-Shock Protein gp96 Enhances T Cell Responses and Protective Potential to Bacillus Calmette-Guerin Vaccine
Heat-Shock Protein gp96 Enhances T Cell Responses and Protective Potential to Bacillus Calmette-Guerin Vaccine
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热休克蛋白 gp96 增强 T 细胞反应和对卡介苗疫苗的保护潜力
DOI：
10.1111/sji.12463
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发表时间：
2016
影响因子：
3.7
通讯作者：
Meng S.
中科院分区：
文献类型：
--
作者：
Ding Y.;Zheng H.;Feng C.;Wang B.;Liu C.;Mi K.;Cao H.;Meng S.
常用的卡介苗（BCG）疫苗仅诱导中度T细胞应答，在成人和老年人群中预防肺结核（TB）的效果较差。因此，开发新的结核病候选疫苗是对抗结核分枝杆菌传播的重要策略。在这里，我们证明了用热休克蛋白gp96作为佐剂免疫导致对BCG疫苗的CD4+和CD8+T细胞应答显著增加。来自gp96免疫小鼠的脾细胞增加了Th1型细胞因子的分泌。此外，加入gp96作为佐剂有效地提高了小鼠对牛分枝杆菌BCG静脉攻击的保护作用。我们的研究揭示了gp96在增强疫苗特异性T细胞应答方面的新特性，以及其作为BCG疫苗抗分枝杆菌感染的佐剂的潜在用途。
The commonly used Bacillus Calmette‐Guérin (BCG) vaccine only induces moderate T cell responses and is less effective in protecting against pulmonary tuberculosis (TB) in adults and ageing populations. Thus, developing new TB vaccine candidates is an important strategy against the spread ofMycobacterium tuberculosis. Here, we demonstrated that immunization with heat‐shock protein gp96 as an adjuvant led to a significantly increased CD4+and CD8+T cell response to a BCG vaccine. Secretion of the Th1‐type cytokines was increased by splenocytes from gp96‐immunized mice. In addition, adding gp96 as an adjuvant effectively improved the protection against intravenous challenge withMycobacterium bovisBCG in mice. Our study reveals the novel property of gp96 in boosting the vaccine‐specific T cell response and its potential use as an adjuvant for BCG vaccines against mycobacterial infection.