Acute exercise remodels mitochondrial membrane interactions in mouse skeletal muscle.
Acute exercise remodels mitochondrial membrane interactions in mouse skeletal muscle.
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DOI：
10.1152/japplphysiol.00819.2013
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Turnbull DM
中科院分区：
文献类型：
--
作者：
Picard M;Gentil BJ;McManus MJ;White K;St Louis K;Gartside SE;Wallace DC;Turnbull DM
哺乳动物细胞中线粒体的一个独特特性是它们能够进行物理相互作用并经历融合/裂变的动态事件，从而重塑它们的形态以及可能的功能。在培养细胞中，与运动过程中发生的代谢扰动类似，会影响线粒体融合和裂变过程，但尚不清楚运动是否会急剧改变体内线粒体形态和/或膜相互作用。为了研究这个问题，我们让小鼠按照正常的身体活动模式接受 3 小时的自愿运动干预，并使用定量电子显微镜方法量化比目鱼肌中的线粒体形态和膜相互作用。单次运动可有效降低血糖（P < 0.05）和肌细胞内脂质含量（P < 0.01），表明肌肉代谢需求增加。运动后，肌膜下线粒体（+116%，P < 0.05）和肌原纤维间线粒体（+191%，P < 0.001）中跨越 Z 线的线粒体数量和相邻线粒体膜之间电子密集接触位点 (EDCS) 的比例立即增加，表明物理相互作用增加。线粒体形态以及线粒体前融合蛋白 Mfn2 和 OPA1 的丰度没有变化。总的来说，这些结果支持了这样的观点，即线粒体膜动力学在骨骼肌中被主动重塑，这可能受到收缩活动和代谢状态的调节。未来的研究需要了解运动和不活动期间线粒体动力学对骨骼肌生理学的影响。
A unique property of mitochondria in mammalian cells is their ability to physically interact and undergo dynamic events of fusion/fission that remodel their morphology and possibly their function. In cultured cells, metabolic perturbations similar to those incurred during exercise influence mitochondrial fusion and fission processes, but it is unknown whether exercise acutely alters mitochondrial morphology and/or membrane interactions in vivo. To study this question, we subjected mice to a 3-h voluntarily exercise intervention following their normal physical activity patterns, and quantified mitochondrial morphology and membrane interactions in the soleus using a quantitative electron microscopy approach. A single exercise bout effectively decreased blood glucose (P < 0.05) and intramyocellular lipid content (P < 0.01), indicating increased muscle metabolic demand. The number of mitochondria spanning Z-lines and proportion of electron-dense contact sites (EDCS) between adjacent mitochondrial membranes were increased immediately after exercise among both subsarcolemmal (+116%, P < 0.05) and intermyofibrillar mitochondria (+191%, P < 0.001), indicating increased physical interactions. Mitochondrial morphology, and abundance of the mitochondrial pro-fusion proteins Mfn2 and OPA1 were unchanged. Collectively, these results support the notion that mitochondrial membrane dynamics are actively remodelled in skeletal muscle, which may be regulated by contractile activity and the metabolic state. Future studies are required to understand the implications of mitochondrial dynamics in skeletal muscle physiology during exercise and inactivity.
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作者：
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DOI：
10.1073/pnas.1014523107
发表时间：
2010-11-02
影响因子：
11.1
作者：
Andrews, Jessica L.;Zhang, Xiping;Esser, Karyn A.
通讯作者：
Esser, Karyn A.