Sensing capabilities of colloidal gold modified with a self-assembled monolayer of a glucose-carrying polymer chain on a glass substrate.
Sensing capabilities of colloidal gold modified with a self-assembled monolayer of a glucose-carrying polymer chain on a glass substrate.
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在玻璃基板上用携带葡萄糖的聚合物链的自组装单层修饰的胶体金的传感能力。
作者：
Shinta Morokoshi;K. Ohhori;Kazuya Mizukami;H. Kitano
用具有引发、链转移和终止作用的N，N-二乙基二硫代氨基甲酰基(N，N-二乙基二硫代氨基甲酰基)衍生物合成了一种含葡萄糖残基的二硫化物聚合物(聚(2-甲基丙烯酰氧乙基-D-吡喃葡萄糖苷))。将二硫键聚合物聚集在胶体金固定的玻璃衬底上，利用紫外可见分光光度计结合表面等离子体共振技术研究了该聚合物作为刀豆蛋白A(ConA)敏感元件的有效性。该传感器对ConA具有浓度依赖性的特异性结合，检测下限为1.9 nM，并且在高离子强度下具有很高的稳定性。本文研究的聚合物涂层器件不仅可用作简单的生物传感器芯片，还有望通过在胶体Au上引入各种功能聚合物来扩展我们对界面现象的了解。
A disulfide-carrying polymer with pendent glucose residues (poly(2-methacryloyloxyethyl D-glucopyranoside)) was obtained by using a benzyl N,N-diethyldithiocarbamoyl derivative which shows the abilities of initiation, chain transfer, and termination (iniferter). The disulfide-carrying polymer was accumulated on a colloidal Au-immobilized glass substrate, and the usefulness of the polymer as a sensing element of concanavalin A (Con A) was examined by using a UV-visible spectrophotometer with the help of surface plasmon resonance. The sensor showed a concentration-dependent specific binding of Con A with a detection limit of 1.9 nM, and furthermore, it had a very high stability at high ionic strength. The polymer-coated device examined here was not only useful as a simple biosensor chip but is also expected to expand our knowledge of interfacial phenomena by introducing various functional polymers on colloidal Au.