Nucleosome Mapping
Nucleosome Mapping
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DOI：
10.1007/978-1-60327-015-1_19
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Gaudreau, Luc
中科院分区：
文献类型：
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作者：
Gevry, Nicolas;Svotelis, Amy;Gaudreau, Luc
核小体是染色质的基本重复单位，它在基因转录过程中起着重要的调控作用。核小体在启动子上的定位是其对包含基因的信号的响应性的重要决定因素。例如，核小体的定位和随后的移动可以调节各种DNA因子对潜在DNA模板的访问。已经提出了几种机制来指导核小体置换的过程，如化学组蛋白修饰，ATP依赖性重塑和组蛋白变体的掺入。因此，染色质不是惰性分子结构，而是高度动态地响应转录过程。在本节中，我们描述了两种方法，允许确定特定基因区域内的确切核小体定位。
The basic repeating unit of chromatin, the nucleosome, is known to play a critical role in regulating the process of gene transcription. The positioning of nucleosomes on a promoter is a significant determinant in its responsiveness to gene-including signals. For example, positioning and subsequent mobilization of nucleosomes can regulate the access of various DNA factors to underlying DNA templates. Several mechanisms have been proposed to direct the process of nucleosome displacement such as chemical histone modifications, ATP-dependent remodelling, and the incorporation of histone variants. Thus, rather than being an inert molecular structure, chromatin is highly dynamic in response to the transcription process. In this section, we describe two methodologies that allow the determination of exact nucleosome positioning within specific gene regions.