On-chip differential interference contrast microscopy using lensless digital holography.
On-chip differential interference contrast microscopy using lensless digital holography.
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DOI：
10.1364/oe.18.004717
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发表时间：
2010-03-01
期刊：
影响因子：
3.8
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Oh C;Isikman SO;Khademhosseinieh B;Ozcan A
我们介绍了使用双折射晶体与无透镜数字全息创建一个片上微分干涉对比（DIC）显微镜。使用具有大孔径的非相干源，创建微物体的同轴全息图，其与单轴晶体和吸收偏振器相互作用，编码芯片上的物体的差分干涉对比度信息。尽管事实上，一个单位的条纹放大率和一个非相干源与一个大的孔径已被使用，全息数字处理这样的全息图迅速恢复的微分相衬图像的试样在一个大的视场~24 mm 2。
We introduce the use of a birefringent crystal with lensless digital holography to create an on-chip differential interference contrast (DIC) microscope. Using an incoherent source with a large aperture, in-line holograms of micro-objects are created, which interact with a uniaxial crystal and an absorbing polarizer, encoding differential interference contrast information of the objects on the chip. Despite the fact that a unit fringe magnification and an incoherent source with a large aperture have been used, holographic digital processing of such holograms rapidly recovers the differential phase contrast image of the specimen over a large field-of-view of ~24 mm2.