32P-postlabeling detection of DNA adducts in fish from chemically contaminated waterways.
32P-postlabeling detection of DNA adducts in fish from chemically contaminated waterways.
复制标题
32P 标记后检测来自化学污染水道的鱼中的 DNA 加合物。
DOI：
10.1016/0048-9697(90)90366-3
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
B. Dunn
中科院分区：
文献类型：
--
作者：
A. Maccubbin;J. J. Black;B. Dunn
鱼是从纽约州布法罗河和密歇根州底特律河被化学污染的地点收集的。这些河流的沉积物含有高水平的化学污染物，包括多环芳烃(PAHs)，来自这些地区的鱼患肝癌的几率很高。为了确定化学物质与DNA的相互作用，以及化学物质作为观察到的肿瘤的原因的可能作用，从肝脏中提取DNA，并将其酶消化成正常的和加合的核苷酸。通过制备性反相高压液相色谱，或通过选择性核酸酶P1去磷酸化正常核苷酸，使DNA消化中富含疏水性、大体积的加合物。然后通过32P-后标记分析对DNA化学加合物进行定量。无论加合物浓缩程序如何，来自污染地区的鱼的DNA色谱图显示一个扩散的、对角线的放射性带，至少部分由多个重叠的离散加合物斑点组成。加合物在对角放射性区域的行为及其未标记的前体与它们被鉴定为各种大体积、疏水性、芳香族遗传毒性化合物的核苷酸加合物是一致的。对胆汁的分析表明，最近接触到了多环芳香化合物。
Fish were collexted from sites in the chemically-contaminated Buffalo River, New York, and the Detroit River, Michigan. The sediments of these rivers have high levels of chemical contaminants, including polycyclic aromatic hydrocarbons (PAHs), and fish from these locations have high prevalences of liver cancer. To determine chemical-DNA interactions and a possible role for chemicals as a cause of the observed tumors, DNA was isolated from livers and was enzymatically digested to normal and adducted nucleotides. The DNA digests were enriched for hydrophobic, bulky adducts, either by preparative reverse phase high pressure liquid chromatography, or by selective nuclease P1 dephosphorylation of normal nucleotides. DNA-chemical adducts were then quantitated by32P-postlabeling analysis. Regardless of the adduct enrichment procedure, the chromatograms derived from DNA of fish from polluted areas showed a diffuse, diagonal radioactive zone consisting, at least in part, of multiple overlapping discrete adduct spots. The behavior of the adducts in the diagonal radioactive zone and of their unlabeled precursors is consistent with their identification as nucleotide adducts of a variety of bulky, hydrophobic, aromatic genotoxic compounds. Analysis of bile demonstrated recent exposure to multi-ringed aromatic compounds.
登录
查看更多内容
膳食暴露和腹腔注射苯并[a]芘对虹鳟鱼的肝癌致癌性。
DOI：
--
发表时间：
1985
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Hendricks,JD;Meyers,TR;Shelton,DW;Casteel,JL;Bailey,GS
通讯作者：
Bailey,GS
由来自β-萘黄酮喂养的虹鳟鱼的细胞色素 P-450 的纯化同工酶催化的苯并[a]芘羟化酶。
影响因子：
5.8
作者：
Williams,DE;Buhler,DR
通讯作者：
Buhler,DR
影响因子：
4.7
作者：
REDDY, MV;GUPTA, RC;RANDERATH, K
通讯作者：
RANDERATH, K
膳食脂肪和苯并芘在小肠中的共同化：使用鳉鱼的吸收模型。
影响因子：
6.5
作者：
Vetter,RD;Carey,MC;Patton,JS
通讯作者：
Patton,JS