Immunocytochemical localization of endopeptidase-24.11 in the nucleus tractus solitarius of the rat brain.
Immunocytochemical localization of endopeptidase-24.11 in the nucleus tractus solitarius of the rat brain.
复制标题
内肽酶 24.11 在大鼠脑孤束核中的免疫细胞化学定位。
DOI：
10.1016/0304-3940(90)90117-r
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发表时间：
1990
影响因子：
2.5
通讯作者：
Bublitz,C
中科院分区：
文献类型：
--
作者：
Lasher,RS;Lutz,EM;Mulholland,F;Sanderson,R;Stewart,JM;Bublitz,C
近年来，有研究认为，内肽酶24.11（EC3.4.24.11）作用于P物质（SP）的N端部分SP（1-7），可能通过作用于孤束核（NTS）细胞，介导压力感受器传入激活的降压效应。在这项研究中，结合的单克隆抗体内肽酶24.11进行了检查，在大鼠大脑的尾髓质水平的化学。通过光学显微镜，强烈的免疫反应被认为是在NTS，在纤维接壤的最后区，并在最后区本身。电镜观察后，内肽酶24.11样免疫反应被认为是与轴突，树突和胶质细胞的细胞骨架和质膜。抗原也与突触前末梢的突触囊泡和质膜相关，主要形成典型的迷走神经传入末梢参与压力感受器反射的轴-树突触。因此，内肽酶24.11似乎是本地化的网站，它可以有效地处理SP之前，其结合到突触后受体。
Recently, it has been hypothesized that the N-terminal portion of substance P (SP), SP(1–7), which results from the action of endopeptidase 24.11 (EC3.4.24.11), could be involved in mediating the depressor effects of baroreceptor afferent activation via its action on cells in the nucleus tractus solitarius (NTS). In this study, the binding of a monoclonal antibody to endopeptidase 24.11 was examined immunohistochemically at the level of the caudal medulla of the rat brain. By light microscopy, intense immunoreactivity was seen in the NTS, in fibers bordering the area postrema, and in the area postrema itself. After electron microscopy, endopeptidase 24.11-like immunoreactivity was seen to be associated with the cytoskeleton and plasma membrane in axons, dendrites and glial processes. Antigen was also associated with synaptic vesicles and plasma membranes in presynaptic terminals forming mainly axo-dendritic synapses typical of vagal afferent terminals involved in the baroreceptor reflex. Thus, endopeptidase 24.11 appears to be localized at sites where it could effectively process SP prior to its binding to postsynaptic receptors.