The tolZ gene of Escherichia coli is identified as the ftsH gene
The tolZ gene of Escherichia coli is identified as the ftsH gene
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大肠杆菌的tolZ基因被鉴定为ftsH基因
DOI：
10.1128/jb.178.12.3457-3461.1996
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发表时间：
1996
影响因子：
3.2
通讯作者：
H. Matsuzawa
中科院分区：
文献类型：
--
作者：
J. Qu;S. Makino;H. Adachi;Y. Koyama;Y. Akiyama;K. Ito;T. Tomoyasu;T. Ogura;H. Matsuzawa
大肠杆菌tolZ突变体耐受大肠杆菌素E2、E3、D、Ia和Ib（Tol-），可以在葡萄糖上生长，但不能在琥珀酸或其他不可发酵的碳源（Nfc-）上生长，并显示温度敏感性生长（Ts）。克隆了一个1.8 kb的DNA片段，补充tolZ突变。对该DNA片段进行测序，发现一个开放阅读框，该阅读框与E. coli FtsH蛋白，一种ATP依赖性金属蛋白酶，与细胞质膜结合。tolZ基因在E. coli基因图谱，并与携带ftsH基因的质粒互补，表明tolZ基因与ftsH基因完全相同。突变的tolZ 21基因也被克隆和测序，并发现有一个单一的碱基变化，造成的氨基酸改变的His-418的酪氨酸在FtsH蛋白。tolZ 21突变体显示出Hfl-（高频率的溶原化）和Std-（停止转移缺陷）表型，这两者都是由于ftsH（hflB）基因中的突变。然而，ftsH 1，ftsH 101和hflB 29突变体没有显示Tol和Nfc表型。tolZ 21突变体被发现具有抑制突变，称为sfhC，其允许细胞存活。在tolZ 21突变体中，单独的sfhC突变不引起Tol-、Nfc-、Ts或Hfl-表型。
Escherichia coli tolZ mutants are tolerant to colicins E2, E3, D, Ia, and Ib (Tol-), can grow on glucose but not on succinate or other nonfermentable carbon sources (Nfc-), and show temperature-sensitive growth (Ts). A 1.8-kb DNA fragment that complemented the tolZ mutation was cloned. The DNA fragment was sequenced, and one open reading frame was found. This frame was identical to a part of the E. coli FtsH protein, an ATP-dependent metalloprotease that binds to the cytoplasmic membrane. The tolZ gene was located at 69 min on the E. coli genetic map, and the mutation was complemented by a plasmid carrying the ftsH gene, indicating that the tolZ gene is identical to the ftsH gene. The mutated tolZ21 gene was also cloned and sequenced and was found to have a single base change that caused an amino acid alteration of His-418 to Tyr in the FtsH protein. The tolZ21 mutant showed Hfl- (high frequency of lysogenization) and Std- (stop transfer-defective) pheno-types, both of which are due to a mutation in the ftsH (hflB) gene. However, the ftsH1, ftsH101, and hflB29 mutants did not show Tol- and Nfc phenotypes. The tolZ21 mutant was found to have a suppressor mutation, named sfhC, which allowed cells to survive. The sfhC mutation alone caused no Tol-, Nfc-, Ts, or Hfl- phenotypes in the tolZ21 mutant.
ATP 酶家族的新成员对于酿酒酵母线粒体呼吸链和 ATP 合成酶复合物的组装至关重要。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tzagoloff,A;Yue,J;Jang,J;Paul,MF
通讯作者：
Paul,MF