Rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease
Rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease
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DOI：
10.1128/iai.00733-06
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发表时间：
2007-04-01
影响因子：
3.1
通讯作者：
Ebersole, Jeffrey L.
中科院分区：
文献类型：
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作者：
Kesavalu, Lakshmyya;Sathishkumar, Sabapathi;Ebersole, Jeffrey L.
人类的主要的多种微生物感染之一在临床上表现为牙周病。牙龈卟啉单胞菌，齿垢密螺旋体，和Tannerella denticola已被强烈暗示为成人牙周炎的病因学中的致病财团的成员。在本研究中，我们假设牙龈卟啉单胞菌，T。denticola和T.连翘在毒力潜力方面具有协同作用，并在大鼠多种微生物感染中诱导慢性牙周炎症，导致牙槽骨吸收。各组大鼠感染牙龈卟啉单胞菌、T. denticola或T.在单一微生物感染中使用连翘，或者在有或没有具核梭杆菌的多种微生物口腔感染中使用所有三个物种。口腔微生物样本的PCR分析表明，感染一种细菌的大鼠在研究期间被每种细菌口腔定殖，血清免疫球蛋白G（IgG）抗体水平升高证实了宿主与感染细菌的相互作用。对多种微生物感染的大鼠进行PCR分析，结果表明，大部分大鼠感染了牙龈卟啉单胞菌、T。denticola和T.作为一个财团，此外，所有大鼠对多微生物聚生体的IgG抗体水平均显著增加。牙槽骨吸收的放射学测量显示，感染有或没有F。与未感染的对照大鼠中的吸收相比，以及与感染一种微生物的大鼠中的吸收相比，nucleatum显示出显著增加的牙槽骨吸收。这些结果表明，牙龈卟啉单胞菌，T。denticola和T.该菌不仅作为与慢性牙周炎相关的聚生体存在，而且还表现出协同毒力，导致牙周炎的免疫炎症性骨吸收特征。
One of the predominant polymicrobial infections of humans is expressed clinically as periodontal disease. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia have been strongly implicated as members of a pathogenic consortium in the etiology of adult periodontitis. In this study we hypothesized that P. gingivalis, T. denticola, and T. forsythia are synergistic in terms of virulence potential and induce chronic periodontal inflammation that leads to alveolar bone resorption in a polymicrobial infection in rats. Groups of rats were infected with either P. gingivalis, T. denticola, or T. forsythia in monomicrobial infections or with all three species in polymicrobial oral infections with or without Fusobacterium nucleatum. PCR analyses of oral microbial samples demonstrated that rats infected with one bacterium were orally colonized by each of the bacteria during the study interval, and increased serum immunoglobulin G (IgG) antibody levels substantiated the interaction of the host with the infecting bacteria. PCR analyses of the rats with polymicrobial infections demonstrated that most rats were infected with P. gingivalis, T. denticola, and T. forsythia as a consortium. Furthermore, all rats exhibited a significant increase in the level of IgG antibody to the polymicrobial consortium. Radiographic measurement of alveolar bone resorption showed that rats infected with the polymicrobial consortium with or without F. nucleatum exhibited significantly increased alveolar bone resorption compared to the resorption in uninfected control rats, as well as the resorption in rats infected with one of the microbes. These results documented that P. gingivalis, T. denticola, and T. forsythia not only exist as a consortium that is associated with chronic periodontitis but also exhibit synergistic virulence resulting in the immunoinflammatory bone resorption characteristic of periodontitis.