In vitro susceptibility studies and detection of vancomycin resistance genes in clinical isolates of enterococci in Nagasaki, Japan
In vitro susceptibility studies and detection of vancomycin resistance genes in clinical isolates of enterococci in Nagasaki, Japan
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日本长崎临床分离肠球菌体外药敏研究及万古霉素耐药基因检测
DOI：
10.1017/s0950268897007954
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发表时间：
1997
影响因子：
4.2
通讯作者：
S. Kamihira
中科院分区：
文献类型：
--
作者：
Y. Hirakata;T. Yamaguchi;K. Izumikawa;J. Matsuda;K. Tomono;M. Kaku;H. Koga;Y. Yamada;S. Kohno;S. Kamihira
肠球菌的糖肽耐药性目前在美国和欧洲引起临床关注。然而，日本肠球菌对万古霉素耐药的详细情况尚不清楚。我们测量了 1995-6 年我院分离的总共 218 株肠球菌临床菌株以及 15 株具有已知耐药基因型标记的菌株的各种抗菌药物的最低抑菌浓度 (MIC)。我们还使用单步多重 PCR 筛选了万古霉素抗性基因。临床分离菌株中，只有两株鸡肠球菌对万古霉素具有中度耐药性（MIC，8 μg/ml），其他菌株均对万古霉素敏感。糖肽（万古霉素和替考拉宁）和链阳菌素（RP 58500 和 RPR 106972）对分离株显示出有效的抗菌作用。此外，氨苄西林对粪肠球菌也有效，而氨苄西林、米诺环素和庆大霉素对鸟肠球菌也有效。未检测到 vanA 或 vanB 基因，而分别从 2 个和 4 个菌株中检测到 vanC1 和 vanC23 基因。我们的结果表明，目前日本 VRE 的发病率估计仍然很低。
Glycopeptide resistance in enterococci is now a cause of clinical concern in the United States and Europe. However, details of vancomycin resistance in enterococci in Japan have been unknown. We measured minimum inhibitory concentrations (MICs) of various antimicrobial agents for a total of 218 clinical strains of enterococci isolated in our hospital in 1995–6 in addition to 15 strains with known genotypic markers of resistance. We also screened vancomycin resistance genes using a single step multiplex-PCR. In clinical isolates, only two strains of Enterococcus gallinarum were of intermediate resistance to vancomycin (MIC, 8 μg/ml), while the others were all susceptible. Glycopeptides (vancomycin and teicoplanin) and streptogramins (RP 58500 and RPR 106972) showed potent antimicrobial effects for the isolates. In addition, ampicillin was also potent for Enterococcus faecalis, while ampicillin, minocycline and gentamicin were potent for Enterococcus avium. No vanA or vanB genes were detected, while vanC1 and vanC23 genes were detected from two and four strains, respectively. Our results suggest that incidence of VRE in Japan may be estimated as still very low at this time.