Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles
Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles
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DOI：
10.1021/nn504778h
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发表时间：
2014-11-01
期刊：
影响因子：
17.1
通讯作者：
Rome, Leonard H.
中科院分区：
文献类型：
--
作者：
Mrazek, Jan;Toso, Daniel;Rome, Leonard H.
核糖体是一种分子机器，在多核糖体复合体中起翻译遗传信息、指导多肽合成和调节新生蛋白质折叠的作用。在这里，我们报告了一个令人惊讶的功能，多核糖体作为一个大的核糖核蛋白复合物，拱顶粒子的系统检查组装的结果。结构和功能证据指向一个拱顶组装模型，其中聚核糖体像3D纳米打印机一样指导多亚基拱顶均聚物的有序翻译和组装，我们将此过程称为聚核糖体模板。基于结构的诱变和体外无细胞表达研究进一步证明了多核糖体在拱顶组装中的重要作用。在拥挤的细胞环境中，聚核糖体模板通过确保生产大型均聚蛋白结构的效率和秩序来防止混乱，并可能解释细胞内许多聚核糖体相关分子组装的起源。
Ribosomes are molecular machines that function in polyribosome complexes to translate genetic information, guide the synthesis of polypeptides, and modulate the folding of nascent proteins. Here, we report a surprising function for polyribosomes as a result of a systematic examination of the assembly of a large ribonucleoprotein complex, the vault particle. Structural and functional evidence points to a model of vault assembly whereby the polyribosome acts like a 3D nanoprinter to direct the ordered translation and assembly of the multi-subunit vault homopolymer, a process which we refer to as polyribosome templating. Structure-based mutagenesis and cell-free in vitro expression studies further demonstrated the critical importance of the polyribosome in vault assembly. Polyribosome templating prevents chaos by ensuring efficiency and order in the production of large homopolymeric protein structures in the crowded cellular environment and might explain the origin of many polyribosome-associated molecular assemblies inside the cell.