Bacterial DNA content in the intestinal wall from infants with necrotizing enterocolitis.
Bacterial DNA content in the intestinal wall from infants with necrotizing enterocolitis.
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DOI：
10.1016/j.jpedsurg.2011.03.026
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发表时间：
2011-06
影响因子：
2.4
通讯作者：
Warner BW
中科院分区：
文献类型：
--
作者：
Bucher BT;McDuffie LA;Shaikh N;Tarr PI;Warner BB;Hamvas A;White FV;Erwin CR;Warner BW
本研究的目的是量化因坏死性小肠结肠炎（NEC）行小肠切除术的新生儿标本中的粘膜细菌DNA。我们从1999年至2008年在我们机构接受手术治疗的所有诊断为NEC的婴儿中获得临床信息和病理标本。从石蜡包埋的手术标本中分离细菌和人类DNA，并使用实时PCR扩增细菌和人类基因。使用线性回归来量化我们的标本中人类和细菌DNA的量。从一组50名婴儿中，我们确定了23名接受手术切除和随后的肠吻合术的婴儿。13名（59%）新生儿患有Bell III期NEC，9名（41%）患有II期NEC。切除标本中的细菌DNA明显多于再吻合标本。这对应于与再吻合标本相比，切除标本中细菌DNA的中位数（IQR）增加1.81（1.11-4.69）倍（p<0.05）。与NEC临床消退后的相同婴儿相比，急性NEC婴儿中的细菌DNA更多。这些发现强调了粘附或侵入性细菌在NEC发病机制中的潜在相关性。
The objectives of our study were to quantify mucosal bacterial DNA within specimens from neonates undergoing small bowel resection for necrotizing enterocolitis (NEC). We obtained clinical information and pathologic specimens from all infants diagnosed with NEC who underwent surgical treatment at our institution from 1999–2008. Bacterial and human DNA were isolated from paraffin-embedded surgical specimens and real-time PCR was used to amplify bacterial and human genes. Linear regression was used to quantify the amount of human and bacterial DNA in our specimens. From a cohort of fifty infants, we identified twenty-three infants who underwent both surgical resection and subsequent intestinal reanastomosis. Thirteen (59%) of the neonates had Bell's Stage III NEC, and nine (41%) had Stage II. There was significantly more bacterial DNA in the resection specimens than in the reanastomosis specimens. This corresponds to a median (IQR) increase of 1.81 (1.11–4.69) fold bacterial DNA in the resection specimen compared to the reanastomosis specimen (p<0.05). There is more bacterial DNA in infants with acute NEC compared with the same infants after the NEC had clinically resolved. These findings underscore the potential relevance of adherent or invasive bacteria across the bowel wall in the pathogenesis of NEC.